BME100 f2013:W900 Group18 L6

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Daniel McDermand Name: Matt Hanson Name: Kirstin Peters Name: student Name: student Name: student [Instructions: add the name of your team's company and/or product here] LAB 6 WRITE-UP Computer-Aided Design TinkerCAD In the lab on November 20th, the TinkerCAD tool was used to make changes to a previous design that was made using the same program. The file in TinkerCAD that we were required to redesign was an image of eight PCR test tubes. The TinkerCAD tool was used by our group to make changes that included adding a band on the tubes that served as a separation between hydrophobic and hydrophilic material, and color coding each one of the tubes in order to eliminate the need for labeling with a sharpie. In general, the TinkerCAD tool is used to create 3D designs, using a variety of shapes, scaling and orienting them appropriately until the desired design is made. The tool also allows changes to previous designs, which, in our case, is what we were able to do using the TinkerCAD program. [Instructions: Show an image of your TinkerCAD PCR tube design here] Implications of Using TinkerCAD for Design The TinkerCAD tool is perfect for practical applications. The designs made in the program are easily transferrable to other programs that are able to print the design. In this case, since the 3D printers available to us use a plastic like material to print an object, the most practical application in this case would be printing one of the plastic components of the OpenPCR system. For example, camera holders could be mass produced using this program for two reasons: the TinkerCAD program is easy to use and it would be very easy to make something as simple as a holder, and the material that is used to print the objects would be sturdy enough to support the camera phone being used when taking pictures of the fluorimeter device. The TinkerCAD program makes it easy to design products in 3D, and the mass production of something as simple as a camera holder would be no problem for the program as well as the printer.] Feature 1: Cancer SNP-Specific Primers [Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.] Background on the cancer-associated mutation [Instructions: Use the answers from questions 3, 4, 5, and 7 to compose, in your own words, a paragraph about rs17879961] Primer design Forward Primer: [Instructions: write the sequence of the forward primer] Cancer-specific Reverse Primer: [Instructions: write the sequence of the forward primer] How the primers work: [Instructions: explain what makes the primers cancer-sequence specific. In other words, explain why the primers will amplify DNA that contains the cancer-associated SNP rs17879961, and will not exponentially amplify DNA that has the non-cancer allele.] Feature 2: Consumables Kit [Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.] [Instructions: IF your consumables packaging plan addresses any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.] Feature 3: PCR Machine Hardware The PCR machine will have more holes for test tubes to get more data in during one sitting. Also, the new PCR machine will have an expanded LED screen to show more information on the test. The PCR tubes will be inside a consumable kit box and each tube will have an hydrophilic bottom and hydrophobic sides to help gather the solution at the bottom of the tube. This is so when the contents are poured out it will not stick to the sides of the tube and effect measurements and data. Also the PCR tubes will be color coded for easier identification because the old method of labeling with markers smudged and smeared off overtime making it easy to misread. Feature 4: Fluorimeter Hardware The main weakness with the Fluorimeter was having the phone stay properly positioned. Our improvement would be to make a new adjustable stand for the phone. This will lock the phone into place so it will not lose focus or move between photos. In addition and adjustable height so it can see any liquid or gel bubble at the proper level. Aldo adding adjustable positioning to tilt the phone to the perfect angle to get the best view and results. Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]