BME100 f2013:W900 Group13 L5: Difference between revisions
Line 69: | Line 69: | ||
|'''Calf Thymus DNA Concentration (Microg/ml)''' ||'''Volume of the 2X DNA Solution (ul)'''||'''Volume of the SYBR GREEN I Assay (ng/mL)'''||'''Final DNA Concentration in SYBR GREEN I Assay (ng/mL)''' | |'''Calf Thymus DNA Concentration (Microg/ml)''' ||'''Volume of the 2X DNA Solution (ul)'''||'''Volume of the SYBR GREEN I Assay (ng/mL)'''||'''Final DNA Concentration in SYBR GREEN I Assay (ng/mL)''' | ||
|- | |- | ||
| 5||80||80||2.5 | | 5||80||80||2.5 | ||
|- | |- | ||
| 2||80||80||1 | | 2||80||80||1 | ||
|- | |- | ||
| 1||80||80||0.5 | | 1||80||80||0.5 | ||
|- | |- | ||
| 0.5||80||80||0.25 | | 0.5||80||80||0.25 | ||
|- | |- | ||
| 0.25||80||80||0.125 | | 0.25||80||80||0.125 | ||
|- | |- | ||
| 0||80||80||blank | | 0||80||80||blank | ||
|} | |} | ||
Revision as of 16:11, 12 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
How the Fluorescence Technique Works The fluorescence technique works by combining a fluorescent dye to the DNA. In this lab, SYBR Green dye was used as the fluorescent dye. The droplets will remain in place due to the Teflon coated glass. A beam of blue light is emitted from the single-drop fluorimeter and is passed through the droplet. A fluorescent green light will be emitted from the droplet which will be measured by taking a picture on a smartphone and analyzing the picture file on ImageJ.
ProcedureSmart Phone Camera Settings
Calibration 1. Adjust the camera settings on the smart phone so that they match the settings above. 2. Place smart phone in the cradle. 3. Make sure that the distance from the smart phone and the cradle is 11 cm. Solutions Used for Calibration
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples
Fitting a Straight Line [Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
|