BME100 f2013:W900 Group12 L5: Difference between revisions
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| [[Image:CourtneyVB.jpg|250px|thumb|Courtney Van Bussum]] | | [[Image:CourtneyVB.jpg|250px|thumb|Courtney Van Bussum]] | ||
| [[Image:NN.jpg|250px|thumb|Nhi Nguyen]] | | [[Image:NN.jpg|250px|thumb|Nhi Nguyen]] | ||
| [[Image: | | [[Image: | ||
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'''Image J Values for All Samples''' | '''Image J Values for All Samples''' | ||
{| {{table}} width=700 | |||
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| align="center" style="background:#f0f0f0;"|'''Calf Thymus DNA concentration (FINAL), microg/ml''' | |||
| align="center" style="background:#f0f0f0;"|'''AREA''' | |||
| align="center" style="background:#f0f0f0;"|'''Mean Pixel Value''' | |||
| align="center" style="background:#f0f0f0;"|'''RAWINTDEN OF THE DROP''' | |||
| align="center" style="background:#f0f0f0;"|'''RAWINTDEN OF THE BACKGROUND''' | |||
|- | |- | ||
| 0||100416||17.7||1777348||78918 | | 0||100416||17.7||1777348||78918 |
Latest revision as of 11:54, 13 April 2014
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
In this lab, a fluorimeter was required to detect fluorescence. This instrument measures how the quantity is related to the amount of florescent material and, less directly, proportional to the amount of the molecule being detected. First, find the smooth side of the glass using gloves. Next, turn on the fluorimeter and place the slide into the fluorimeter with the smooth side down. Set the camera timer to 3 seconds and place the phone on the cradle. Adjust the height of the fluorimeter so that the camera views the slide nearly on the edge. Place a 80 uL drop of SYBR Green I solution in the middle of the first 2 circles on the slide. Then place the same amount of the Sample Calibration solution on top of the SYBR Green solution. Adjust the slide so that the light illuminates the center of the drop. Make sure to adjust the distance between the smartphone and fluorimeter to greater than 4cm away and record the distance. Keeping the drop in focus, cover with the light box keeping one flap open. Initiate the timer and close the flap before the picture is taken. Remove the drop from the slide using the micropipette and then repeat the same procedure with the next two clear circles for each sample calibration solution.
Solutions Used for Calibration
Instructions
Data AnalysisRepresentative Images of Samples Sample Image with no DNA
Image J Values for All Samples
Fitting a Straight Line
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