BME100 f2013:W900 Group12 L5: Difference between revisions
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'''Representative Images of Samples''' | '''Representative Images of Samples''' | ||
'' | ''[[Image:Example with no DNA.jpg]]] | ||
Sample Image with no DNA | |||
Sample Image with no DNA'' | |||
'' | ''[[Image:Sample with DNA.jpg]]] | ||
Sample Image with DNA (positive signal) | |||
Sample Image with DNA (positive signal)'' | |||
Revision as of 14:42, 12 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
In this lab, a fluorimeter was required to detect fluorescence. This instrument measures how the quantity is related to the amount of florescent material and, less directly, proportional to the amount of the molecule being detected. First, find the smooth side of the glass using gloves. Next, turn on the fluorimeter and place the slide into the fluorimeter with the smooth side down. Set the camera timer to 3 seconds and place the phone on the cradle. Adjust the height of the fluorimeter so that the camera view of the slide nearly edge on. Place a 80 uL drop of SYBR Green I solution in the middle of the first 2 circles on the slide. Then place the same amount of the Sample Calibration solution on top of the SYBR Green solution. Adjust the slide so that the light illuminates the center of the drop. Make sure to adjust the distance between the smartphone and fluorimeter to greater than 4cm away and record the distance. Keeping the drop in focus, cover with the light box keeping one flap open. Initiate the timer and close the flap before the picture is taken. Remove the drop from the slide using the micro pipette and then repeat the same procedure with the next two clear circles for each sample calibration solution.
[Instructions: See worksheet page 6.]
Data AnalysisRepresentative Images of Samples Sample Image with no DNA Sample Image with DNA (positive signal)
Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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