BME100 f2013:W900 Group12 L5: Difference between revisions
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'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
The single-drop fluorimeter device is a box that emits a blue light. A slot is present on the device for a sample slide to be placed on. The surface of the sample slide on which the solution can be dropped on is hydrophobic, and because of this water (when dropped on the surface) forms a sphere which can be observed for testing the presence of DNA. Then the SYBR Green dye and DNA solutions are placed on the sample slide, and blue light passes through the solution so that green light will be emitted. This process enables users to measure DNA in | The single-drop fluorimeter device is a box that emits a blue light. A slot is present on the device for a sample slide to be placed on. The surface of the sample slide on which the solution can be dropped on is hydrophobic, and because of this water (when dropped on the surface) forms a sphere which can be observed for testing the presence of DNA. Then the SYBR Green dye and DNA solutions are placed on the sample slide, and blue light passes through the solution so that green light will be emitted. This process enables users to measure DNA in fluorescent materials.<br> | ||
[[Image:Singledrop.jpg|500px|thumb|Single-Drop Fluorimeter]] | |||
Revision as of 11:29, 6 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]
Calibration [Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[Instructions: See worksheet page 6.]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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