BME100 f2013:W1200 Group9 L6

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Salvador Avina Name: Rachael Hall Name: Kenna Lum Name: Michelle Sigona Name: Jessica Stradford [Instructions: add the name of your team's company and/or product here] LAB 6 WRITE-UP Computer-Aided Design TinkerCAD TinkerCAD is a useful tool that allows us to create a product or take a product already created and edit it to create a new design before physically creating the product. This tool allows us to visualize what we want to add or change on the product and we can easily see any problems that we would run into without having creating the product yet. In lab, this tool helped us take the already created TinkerCAD design of the tubes we had used for our PCR samples and we made our own changes to it to make them more useful. Implications of Using TinkerCAD for Design 'The vast range of shapes, colors, numbers, and designs was used so that it is easy to correclty label the tubes for the PCR machine. Our group took what we already knew about the tubes. which is that they are small and compact, and added our own ideas and extras to it and made it our own. We color coded the tubes, so that less writing would have to be done on the actual tube to label them. It is hard to write, and then read writing so small, and it is easy to make mistakes that way' Feature 1: Cancer SNP-Specific Primers [Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.] Background on the cancer-associated mutation [Instructions: Use the answers from questions 3, 4, 5, and 7 to compose, in your own words, a paragraph about rs17879961] Primer design Forward Primer: [Instructions: write the sequence of the forward primer] Cancer-specific Reverse Primer: [Instructions: write the sequence of the forward primer] How the primers work: [Instructions: explain what makes the primers cancer-sequence specific. In other words, explain why the primers will amplify DNA that contains the cancer-associated SNP rs17879961, and will not exponentially amplify DNA that has the non-cancer allele.] Feature 2: Consumables Kit The packaging of our consumables will be very similar to the way they are currently packaged. The plastic tubes will come in rows of sixteen, eight tubes on each side (as shown in the image above). These tubes will come in cardboard packages and will hold 240 tubes per package. each package will include a rack for storing the tubes. Two racks holding 120 tubes each will be included with each package of 240 tubes. The micropipeter will be packaged separately and will include 200 plastic tips with purchase. These tips will be racked in a plastic rack and extra tips will also be available for purchase in quantities of 50 and 100. The PCR mix and the primers will also be packaged together. All of the items in the consumables kit are available as one package for a set price and are also available for separate sale. Our group decided to do this so that consumers could buy all the necessary items they needed at one reasonable price or they could buy separate items if they only needed a few individual things. One of the problems that our group mentioned was the lack of labeling on the plastic tubes. The tubes that we designed on TinkerCad are color coated in order to help differentiate the different primers, and DNA samples. The packages of tubes will also come with small labeling strips so that consumers will be able to label their specific tubes and have some way to keep their samples organized. Feature 3: PCR Machine Hardware The PCR will be used in our system as the main component. It will be self-opperatable with the use of the computer, as well as easy to construct. It will contain all the necessary parts within the package, such as the fan and PCR racks. The boc will be light enough to carry, and sturdy enough to withstand movement from one location to another. It will be similair to the portable PCR used in the lab, however there will be one small difference. Our PCR machine will be redesigned to be easier to open. The top of the other machine was very difficult to open in order to insert the samples. However, in the new design the top will open "Automaticaly" with the push of a button. By pushing the button, the PCR machine's top latch will pop open, so that all one has to do to open the machine is lightly lift. Feature 4: Fluorimeter Hardware [Instructions: Summarize how you will include the fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really REALLY awesome and easy to score.] [Instructions: IF your group has decided to redesign the fluorimeter to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.] Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]