BME100 f2013:W1200 Group5 L6: Difference between revisions
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'''TinkerCAD'''<br> | '''TinkerCAD'''<br> | ||
'' | ''TinkerCAD is an online computer aided design tool''<br> | ||
''[Instructions: Show an image of your TinkerCAD PCR tube design here]'' | ''[Instructions: Show an image of your TinkerCAD PCR tube design here]'' |
Revision as of 20:58, 26 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAMLAB 6 WRITE-UPComputer-Aided DesignTinkerCAD TinkerCAD is an online computer aided design tool [Instructions: Show an image of your TinkerCAD PCR tube design here]
Feature 1: Cancer SNP-Specific PrimersBackground on the cancer-associated mutation
How the primers work: The reverse primer will bind to a complementary cancer-SNP containing template that was taken from a patient sample. The reverse primer only binds to the complementary sequence if it 100% matches just as the forward primer will also bind to its complement given it matches 100%. When the primer does not match the sequence completely, it will not be replicated. This makes it so only the DNA with the cancer-associated SNP will be replicated and any DNA that has the non-cancer allele will not be replicated.
Feature 2: Consumables KitThe Eazee Baike PCR system comes complete with a micropipettor, pipette tips, PCR sample tubes, PCR mix, and primers. In addition to having all ingredients of the experiment readily available, the PCR tubes come pre-labeled and sorted which allows the user to begin the experiment immediately after opening the package. Other kits force the user to separate the tubes from large connected groups, down to a manageable size while also requiring the user to label the individual tubes. We find that pre-labeling and grouping the PCR tubes can save the user time and hassle.
Feature 3: PCR Machine HardwareThe Eazee Baike PCR machine is nearly identical in design to the Open PCR machine used in BME lab. The main design change happens internally, in the PCR Machine software. Eazee Baike PCR's new software will ensure that all cycles run through the machine are uniform in both time and temperature. This means that the user simply has to put the PCR tubes into the machine and start it. The machine will take care of the rest, displaying a time of completion so the user knows how long to wait. In addition, the PCR machine will include a USB drive which will allow the user to either connect the machine to a computer, or download the data onto a flash drive (flash drive included in kit).
Feature 4: Fluorimeter HardwareThe Eazee Baike PCR Fluorimeter is designed with a smartphone compatible dock that is attached to the base at a fixed position. This will reduce drop analysis error due to varied camera distances. There will also be an insert included with the kit that gives instructions on how to download the Eazee Baike PCR Camera App to your smartphone. This app is customized to take pictures in the dark and allows the user to manually focus the camera which will reduce errors related to camera quality.
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.] |