BME100 f2013:W1200 Group13 L6: Difference between revisions
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==Feature 4: Fluorimeter Hardware== | ==Feature 4: Fluorimeter Hardware== | ||
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<br>The fluorimeter is used to test for cancer in a sequence. A camera phone is used to take a picture of the florescence of the SYBR green dye mixed with PCR sample. The setup of the fluorimeter includes a stand for the camera, which helps the camera stay upright to take the picture, a black box to cover the phone stand and the dye. The black box blocks out light so results aren't affected.<br> | <br>The fluorimeter is used to test for cancer in a sequence. A camera phone is used to take a picture of the florescence of the SYBR green dye mixed with PCR sample. The setup of the fluorimeter includes a stand for the camera, which helps the camera stay upright to take the picture, a black box to cover the phone stand and the dye. The black box blocks out light so results aren't affected.<br> | ||
Revision as of 23:09, 26 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR COMPANY
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD Our group used TinkerCAD to create new PCR tubes. The design of the tubes was created while considering organization and ease of handling. The openPCR machine that we used in class is set up to hold four rows of four. We set up our tubes to be in two groups, Each group connected for handling and organization. The first group is the + control and the three trials of patient 1, thus our tubes are labeled so "+" "1-1" "1-2" "1-3" are visible and have a slight grade so they can be felt as well without getting stuck in the machine. The same was done for the second group of tubes with the - control and patient2's trials respectively.
TinkerCAD could be used practically for this experiment because the design for the OpenPCR machine could be redesigned. The machines that we used had several issues. These issues revolved around overheating and user construction flaws. With these flaws in mind the way we would redesign the machine would be to print the shell of the machine a little bigger, then repositioning the fan so it has two parts, one blowing cold air in and the other moving hot air out. On top of that the heat sink should be moved next to the hot air outtake fan so it is constantly cooled and displacing heat. This will help the overheating issue. The next thing could be to print the machine with small latches or pins because the screws made a screw driver necessary and also cause issues with missing pieces and splitting wood. With each printed perhaps with an L hook and a button the machines would all be put together identically, thus eliminating the user construction problem.
Feature 1: Cancer SNP-Specific PrimersBackground on the cancer-associated mutation
Primer design
How the primers work: Primers are custom pieces of DNA that are made to attach at the top strand of DNA and also at the end of the specific segment of DNA. The two strands are not identical, but they are complimentary.
Feature 2: Consumables KitPackaging of consumables kit
Feature 3: PCR Machine HardwareThe PCR machine will be packaged separately from the box of consumables that way it does not damage any of the items. They can be bought together in a complete package but they will not be stored together to avoid any damage to the PCR machine or the consumables. The tube holders in the PCR machine would have a stopper in the corner to make sure that the lid is on all the way and not breaking the tubes. This is a simple modification that can create more accuracy. The stopper would be the slightly higher than the tubes but not enough to make the tubes not touch the lid. It is higher enough to take the pressure from the lid off the tubes, but not enough to effect the experiment.
Feature 4: Fluorimeter Hardware
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.] |