BMCB625:ncRNA: Difference between revisions

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(New page: {{Template:BMCB625}} <div style="padding: 10px; width: 720px; border: 5px solid #B3CD4E;"> ==(Homework) Questions== ===Larry=== ---- ===Chayne=== ---- ===Jon=== ---- ===Chris=...)
 
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===Mahta===
===Mahta===


Q1.  From the data can you envision a model in which several RNAs similar to BC1 act as "bridges" or "adaptors" between FMRP and mRNAs targetd for degradation?


Q2.  High salt is needed to efficiently immunoprecipitate FMRP (the authors state that the C-terminal can be masked at physiological concentrations).  Also, the recombinant FMRP is only stable in 1M salt.  Is this troublesome?


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Revision as of 07:28, 12 June 2007

BMCB625 Advanced Topics in Molecular Biology

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(Homework) Questions

Larry


Chayne


Jon


Chris


Mahta

Q1. From the data can you envision a model in which several RNAs similar to BC1 act as "bridges" or "adaptors" between FMRP and mRNAs targetd for degradation?

Q2. High salt is needed to efficiently immunoprecipitate FMRP (the authors state that the C-terminal can be masked at physiological concentrations). Also, the recombinant FMRP is only stable in 1M salt. Is this troublesome?