BMBMethod:Protocols

<owwmenu font="arial, helvetica, sans-serif" bold="1" color="black" bgcolor="white" hovercolor="black" bghovercolor="gray" topFontSize="10" fontSize="8" pagewidth="650" image="OldMain2.jpg" lab="BMBMethod"> Home= Meetings=#,Schedule=Schedule, Past=Past, Presentations=Presentations Protocols=Protocols, Protein=Protocols#Protein, DNA=Protocols#DNA, RNA=Protocols#RNA, Other=Protocols#Other Tips & Tricks=TipsandTricks, Protein=TipsandTricks#Protein, DNA=TipsandTricks#DNA, RNA=TipsandTricks#RNA, Other=TipsandTricks#Other Equipment=Equipment, Departmental=Equipment#BMBDepartment, Personal=Equipment#Personal, Other=Equipment#Other Reagents=Reagents, Departmental=Reagents#Departmental, Cheap Buys=Reagents#Cheap Reagents </owwmenu>

Protein

Electrophoresis

• Poly-Acrylimide Gel Electrophoresis (PAGE)
• Blue-Native PAGE (BN-PAGE)
• Tris-Acetate PAGE

Protein Assay

• Modified Lowry Assay
• Bradford Assay

Expression

• Bacterial Expression of Proteins
• Cell-free expression of Proteins

Purification

• Immobilized Metal Anion Chromatography (i.e. Nickel-NTA)

Staining

• Coomassie
• Silver
• Heme Staining
• Immuno-detection (western blotting)

Antibodies

• Generation of Polyclonal Antibodies
• Generation of Monoclonal Antibodies
• Purification of Antibodies using Sulfo-Link resin
• Purification of Antibodies using Membrane Elution

Other

• TCA Precipitation of Proteins
• Acetone Precipitation of Proteins
• Ethanol Precipitation of Proteins

DNA

Electrophoresis

• BMBMethod:Super-fast DNA Electrophoresis

Cloning

• Restriction Digestion
• Ligation
• InFusion
• Gibson Assembly
• SLiCE
• Ligation-Independant Cloning
• BioBlocks
• Site Directed Mutagenesis of Plasmids

Genome Editing

• CRISPER/Cas9
• something else

Purification

• Alkaline Lysis plasmid mini-prep procedure
• Re-use of commercial DNA spin columns
• Ethanol Precipitation of DNA
• Phenol-chloroform extraction of DNA

Other

• Polymerase Chain Reaction (PCR)
• One-step DNA extraction from Arabidopsis thaliana (for PCR)

RNA

• Real-Time PCR for transcript quantification
• Working with RNA

Other

• Creation of highly-competent (chemical) E. coli
• Heat-shock transformation of DNA into E. coli
• Transformation of DNA into Agrobacterium
• Floral Dip transformation of DNA into Arabidopsis thaliana
• Heat-shock transformation of DNA into E. coli (Fisher, M.J.)