BMBMethod:Protocols: Difference between revisions
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* [[BMBMethod:AcetonePrecip|Acetone Precipitation of Proteins]] | * [[BMBMethod:AcetonePrecip|Acetone Precipitation of Proteins]] | ||
* [[BMBMethod:EtOHProtein|Ethanol Precipitation of Proteins]] | * [[BMBMethod:EtOHProtein|Ethanol Precipitation of Proteins]] | ||
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===Other=== | ===Other=== | ||
* [[BMBMethod:PCR|Polymerase Chain Reaction (PCR)]] | * [[BMBMethod:PCR|Polymerase Chain Reaction (PCR)]] | ||
* [[BMBMethod:ArabiDNA|One-step DNA extraction from Arabidopsis thaliana (for PCR)]] | |||
Revision as of 11:12, 29 October 2014
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Protein
Electrophoresis
Protein Assay
Expression
Purification
Staining
Antibodies
- Generation of Polyclonal Antibodies
- Generation of Monoclonal Antibodies
- Purification of Antibodies using Sulfo-Link resin
- Purification of Antibodies using Membrane Elution
Other
DNA
Electrophoresis
Cloning
- Restriction Digestion
- Ligation
- InFusion
- Gibson Assembly
- SLiCE
- Ligation-Independant Cloning
- BioBlocks
- Site Directed Mutagenesis of Plasmids
Genome Editing
Purification
- Alkaline Lysis plasmid mini-prep procedure
- Re-use of commercial DNA spin columns
- Ethanol Precipitation of DNA
- Phenol-chloroform extraction of DNA
Other
