BMBMethod:Equipment

<owwmenu font="arial, helvetica, sans-serif" bold="1" color="black" bgcolor="white" hovercolor="black" bghovercolor="gray" topFontSize="10" fontSize="8" pagewidth="650" image="OldMain2.jpg" lab="BMBMethod"> Home= Meetings=#,Schedule=Schedule, Past=Past, Presentations=Presentations Protocols=Protocols, Protein=Protocols#Protein, DNA=Protocols#DNA, RNA=Protocols#RNA, Other=Protocols#Other Tips & Tricks=TipsandTricks, Protein=TipsandTricks#Protein, DNA=TipsandTricks#DNA, RNA=TipsandTricks#RNA, Other=TipsandTricks#Other Equipment=Equipment, Departmental=Equipment#BMBDepartment, Personal=Equipment#Personal, Other=Equipment#Other Reagents=Reagents, Departmental=Reagents#Departmental, Cheap Buys=Reagents#Cheap Reagents </owwmenu>

BMBDepartment

The BMB Department has a variety of equipment that it owns, all of which are available for use by BMB students

Frear

• Autoclaves
  • 270 N. Frear - 2 (Digital)
  • 301 S. Frear - 2 (Digital)
  • 435 S. Frear - 1 (Turn dial)
  • 437 S. Frear - 2 (Turn Dial)
• Beckman Ultracentrifuge - 312 S. Frear (new)
  • 70Ti Rotor
  • SW-32 Rotor & 38.5mL Thinwall tubes
• Beckman Ultracentrifuge - 320 S. Frear (1/2 department, old, sometimes has issues)
• Beckman Analytical Ultracentrifuge - 312 S. Frear
• Shaker - 321 S. Frear
• Darkroom - 435A S. Frear
  • Film Developer
  • Transilluminator w/Geldoc
• Typhoon Scanner 8600 - 436 S. Frear
• Ovens (2) - 437 S. Frear
  • Large & Old, up to 550F
  • Fischer Isotemp

Althouse

• Autoclaves
  • 4 Althouse - 1
  • 207 Althouse - 1
  • 307 Althouse - 2 (one older than time itself)
  • 408 Althouse - 2
• Dry Ice - 4 Althouse
• Typhoon 9410 - 206 Althouse
• Scintillation Counters (2) - 206 Althouse
• -80 Freezer - 206 Althouse
• Darkroom - 303A Althouse
  • Dip-tank development
  • Transilluminator
  • Controlled by Dr. Postle?
• Glassware Dryer - 307 Althouse
• French Press (Piston only) - 307 Althouse
  • French Pressure Cell available from the below labs
  • Cameron Lab
  • Postle Lab (I think you can borrow this)

Personal

If you lab has equipment that would be available to other members of the department, please post here.

Tien Lab

307 S. Frear

• 96 well plate spectrophotometer
  • Quartz 96-well plate for UV measurements
  • Can do both end-point and kinetic (time-course) measurements
• Bio-rad gel equipment
  • Plates and combs for 0.75, 1, and 1.5mm thickness minigels
  • Combs to make 2nd Dimension mini-gels
  • Large scale (16x20cm) gel running and transfer equipment
  • Multi-gel (13 mini gels) casting unit
• Gradient makers for making sucrose or PAGE gradients
• Gel dryer, suitable for Radioactive samples
• Coy glove box
• RC3B Plus Superspeed centrifuge
  • H-1000B Swinging bucket rotor (50mL and 15mL falcon tubes)
  • Microplate rotor for 96-well plates
• 8- and 12-channel Pipettes

Cameron Lab

201 & 206 Althouse

• Nanodrop - 206 Althouse
• Eagle Eye II gel doc - 206 Althouse
• French Press Cell - 201 Althouse

Other

There are several Penn State facilities that offer a variety of services and instrumentation (for a fee, of course). Below is a selection of what we have available to us

HUCK

http://www.huck.psu.edu/facilities

X-Ray Crystallography Facility - 8 Althouse

• Protein Crystallization
• Dynamic Light Scattering
• Small-angle X-ray scattering (SAXS)

Automated Biological Calorimetry Facility - 8 Althouse

• Isothermal Titration Calorimetry (ITC)
• Differential scanning calorimetry
• Protein and lipid quantification

Metabolomics Core Facility - 319 Life Sciences

• Multiple liquid chromatography-mass spectrometry (LC-MS) devices for quantification of metabolites

Microscopy and Cytometry Facility - N-032 & W-124A MSC

• Electron Microscopy
  • Critical point dryers
  • Sputter coater
  • Microtomes (including cryo- and parrifin-)
  • High-pressure freezer
• Flow Cytometry
• Cell sorting
• Fluorescence and confocal microscopy
• Laser-capture microdissection

Proteomics and Mass Spectrometry Core Facility - 3 Althouse

• LC-MS/MS for protein identification
• 2D gel electrophoresis (including DIGE)
• Liquid/Gas Chromatography-Mass Spectrometry (LC-MS or GC-MS) method development
• Molecular mass determination of small molecules

Genomics Core Facility - 4th Floor Chandlee

• Microarray analysis
• Next-gen sequencing
• Sanger sequencing
• Real-Time PCR (qPCR)
• Genotyping
• Oligonucleotide synthesis

Materials Characterization Lab (MCL)

North Basement of MSC, mostly http://www.mri.psu.edu/facilities/mcl/index.asp

• Field Emission Auger Electron Spectroscopy (AES)
• Atomic Force Microscopy (AFM)
• Electron Backscattered Diffractometry (EBSD)
• Energy Dispersive Spectroscopy (EDS)
• Fourier Transform Infrared Spectroscopy (FTIR)
• Confocal Raman
• Scanning Electron Microscopy (SEM)
• Transmission Electron Microscopy - TEM
• X-Ray Photoelectron Spectroscopy

Hershey

http://www.huck.psu.edu/facilities/facilities/find-by-location/hershey

• Flow Cytometry
• Sanger sequencing
• qPCR
• Next-gen sequencing
• Peptide synthesis
• MicroCT Scanner
• Confocal microscopy
• CryoEM
• Transmission EM
• Mass spectrometry of small molecules and proteins
• X-ray crystallography

Fermentation Facility

57 Fenske http://www.huck.psu.edu/facilities/fermentation-up

• Microfluidizer for lysis of large volumes of cells
• Fermenters for growing large batches of cells (30/80/150/300L)