BISC220/S14: Mod 1 Media: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: {{Template:BISC220/S14}} <div style="padding: 10px; width: 720px; border: 5px solid #33ccff;"> == β-galactosidase Series Media Recipes == {| border="1" |+ ! Solution !! Composition |...) |
No edit summary |
||
| Line 1: | Line 1: | ||
{{Template:BISC220/S14}} | {{Template:BISC220/S14}} | ||
<div style="padding: 10px; width: 720px; border: 5px solid #33ccff;"> | <div style="padding: 10px; width: 720px; border: 5px solid #33ccff;"> | ||
<div class=noprint> | |||
[[BISC220/S14: Mod 1 Lab 1 | Lab 1: Introduction to BISC220 Lab]]<br> | |||
[[BISC220/S14: Mod 1 Lab 2 | Lab 2: Induction of β-galactosidase and Molecular Modeling]]<br> | |||
[[BISC220/S14: Mod 1 Lab 3 | Lab 3: Affinity Chromatography and Polyacrylamide Gel Electrophoresis]]<br> | |||
[[BISC220/S14: Mod 1 Lab 4 | Lab 4: Specific Activity Determination]]<br> | |||
[[BISC220/S14: The Michaelis-Menten Model | The Michaelis-Menten Model]]<br> | |||
[[BISC220/S14: Mod 1 Lab 5 | Lab 5: Enzyme Kinetics]]<br> | |||
[[BISC220/S14: Mod 1 Media | Media Recipes]]<br> | |||
[[BISC220/S14: Mod 1 Science Writing | Science Writing and Figure Design]]<br> | |||
</div> | |||
== β-galactosidase Series Media Recipes == | == β-galactosidase Series Media Recipes == | ||
{| border="1" | {| border="1" | ||
Latest revision as of 07:43, 5 June 2013
β-galactosidase Series Media Recipes
| Solution | Composition |
|---|---|
| Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar (only in plates) |
| Ampicillin | Stock = 25 mg/ml in water |
| IPTG (Isopropyl β-D-1-thiogalactopyranoside) | Stock 1 mg/ml |
| Na2CO3 | Stock 1M |
| Z-buffer | 60 mM Na2HPO4 60 mM NaH2PO4 1 mM MgSO4 10 mM HCl 0.27% β-mercaptoethanol |
| ONPG (ortho-Nitrophenyl-β-galactoside) | Stock 4 mg/ml |
| Laemmli Solution (2x Sample Buffer) | 0.125 M Tris, pH 6.7 4% SDS 20% glycerol 10% β-mercaptoethanol |
| Gel Running Buffer | 0.92 M Glycine 0.05 M Tris Base 0.1% SDS |
| GelCode Blue Stain Reagent | Thermo Scientific |
