BISC220/S13: Media Recipes: Difference between revisions
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Tucker Crum (talk | contribs) (New page: {{Template:BISC220/S13}} <div style="padding: 10px; width: 720px; border: 5px solid #E05D1B;"> == Apoptosis Series Media Recipes == {| border="1" |+ ! Solution !! Composition |- ! PBS ...) |
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! anti-PARP-1 antibody (mouse) | ! anti-PARP-1 antibody (mouse) | ||
| diluted 1:1000 | | diluted 1:1000 | ||
|- | |||
! Immun-Star™ WesternC™ Chemiluminescence Kit | |||
| www.bio-rad.com <br> Catalog #170-5070 | |||
|- | |||
! Precision Plus Protein™ WesternC™ Standards (Ladder) | |||
| www.bio-rad.com <br> Catalog #161-0385 | |||
|- | |- | ||
|} | |} | ||
Latest revision as of 11:16, 8 April 2013
Apoptosis Series Media Recipes
| Solution | Composition |
|---|---|
| PBS (Phosphate Buffered Saline) 1X | 2.68 mM KCl 1.46 mM KH2PO4 136.9 NaCl 8.1 mM Na2HPO4 |
| PBST (PBS with Tween 20) | 1X PBS with 0.25% Tween 20 |
| Trypsin | Stock = 0.25% |
| Cell Culture Media | 1 Packet DMEM Powder 0.15% NaHCO3 10% Newborn Calf Serum |
| Trypan Blue | 0.1% Trypan Blue in water |
| Etoposide | 100 mM in DMSO (stock) 500 μM (effective) |
| DNA extraction buffer | 50 mM Tris pH 8.0 20 mM Na2EDTA 10 mM NaCl 1% SDS 20 ug/ml RNase A 100 ug/ml Proteinase K |
| PMSF/glucose buffer (protein buffer 1) | 50 mM glucose 25 mM Tris pH 8.0 10 mM Na2EDTA and 1 mM PMSF |
| urea/SDS buffer (protein buffer 2) | 50 mM Tris pH 6.8 6M urea 6% B-ME 3% SDS 0.003% bromophenol blue |
| anti-PARP-1 antibody (mouse) | diluted 1:1000 |
| Immun-Star™ WesternC™ Chemiluminescence Kit | www.bio-rad.com Catalog #170-5070 |
| Precision Plus Protein™ WesternC™ Standards (Ladder) | www.bio-rad.com Catalog #161-0385 |
