BISC219/F13: RNAi Lab 7

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(New page: {{Template:BISC219/F13}} <div style="padding: 10px; width: 720px; border: 5px solid #FF6600;"> == Lab 7: Series 3 - Examining the effect of heat shock on the CL2070 strain == '''Instruc...)
(Lab 7: Series 3 - Examining the effect of heat shock on the CL2070 strain)
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'''Instructors will do 24 hours before lab:'''<br>
'''Instructors will do 24 hours before lab:'''<br>
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#Your instructor or our lab specialist will come in 24 hours before lab and "heat shock" one plate each of your worms that have been incubating at 15°C and 23°C.  The second plate at each temperature serves as your control.  Heat shocking involves moving the worms to a 37°C incubator for 30-45 minutes.  The worms will then be placed back at their proper temperature until you use them tomorrow.<br><br>
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Your instructor or our lab specialist will come in 24 hours before lab and "heat shock" one plate each of your worms that have been incubating at 15°C and 23°C.  The second plate at each temperature serves as your control.  Heat shocking involves moving the worms to a 37°C incubator for 30-45 minutes.  The worms will then be placed back at their proper temperature until you use them tomorrow.<br><br>
'''To Do in Lab Today'''<br>
'''To Do in Lab Today'''<br>

Revision as of 14:38, 26 August 2013


Lab 7: Series 3 - Examining the effect of heat shock on the CL2070 strain

Instructors will do 24 hours before lab:
Your instructor or our lab specialist will come in 24 hours before lab and "heat shock" one plate each of your worms that have been incubating at 15°C and 23°C. The second plate at each temperature serves as your control. Heat shocking involves moving the worms to a 37°C incubator for 30-45 minutes. The worms will then be placed back at their proper temperature until you use them tomorrow.

To Do in Lab Today
We only have one fluorescent compound scope to view the worms and one fluorescent dissecting scope. While some groups are scoring their worms others will be working with the instructor in the microscope room to view and photograph their worms.

  1. Bring your 4 plates of worms down to the microscope room.
  2. You will first look at your worms and take some pictures under the dissecting scope. Place one of your non-heat shocked plate on the stage of the dissecting scope and expose the worms to the UV light. Record in your notebook what you see. Are the worms glowing? What part of the worms are glowing?
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