BISC219/F13: Media Recipes: Difference between revisions
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(New page: {{Template:BISC219/F13}} <div style="padding: 10px; width: 720px; border: 5px solid #FF6600;"> == Ingredients and Stock Concentrations for some of the reagents used in Series 3 == {| bord...) |
(New page: {{Template:BISC219/F13}} <div style="padding: 10px; width: 720px; border: 5px solid #FF6600;"> == Ingredients and Stock Concentrations for some of the reagents used in Series 3 == {| bord...) |
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Latest revision as of 10:47, 20 August 2013
Ingredients and Stock Concentrations for some of the reagents used in Series 3
| Reagent | Composition |
|---|---|
| Luria-Bertoni Medium (LB) | 1% Tryptone 0.5% Yeast Extract 1% NaCl (2% agar in plates) |
| Nematode Growth Medium Lite(NGM lite)- Available in powder form from US Biological Cat. #N1005 (http://www.usbio.net) | 0.3% NaCl 1.7% Bacto-agar 0.25% Bacto-peptone 0.1% (vol/vol)cholesterol |
| Ampicillin* | Stock = 50 mg/ml in water use at 1:1000 in liquid cultures use at 1:500 in LB amp plates |
| Tetracycline* | Stock = 12.5 mg/ml in water use at 1:1000 in liquid cultures and in solid medium plates |
| IPTG | Stock = 0.5 M use 0.5 mM in cultures |
| 1x PBST (phosphate buffered saline with Tween20) | 137 mM NaCl,2.7 mM KCl,4.3 mM Na2HPO4,1.47 mM KH2PO4, 0.25% -Adjust to a final pH of 7.4. |
*NOTE: These concentrations are STOCK concentrations - not effective concentrations!
