BISC219/F13: Assignment Series2 Outline Summary

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Assignment: Series 2 Summary/ Diagram & Explain Linkage Analysis

Your assignment due at the beginning of Lab 3 is in two parts. Part 1 is to summarize concisely the Series 2 project in classical (forward genetics). The Background information at Description of the Project in Series 2 should be helpful but remember to summarize this extensive explanation and use your own words! The goal of this part of the assignment is for you to have a complete and clear understanding of the broad topic, our investigative goals, and the experimental approach that we will use to accomplish these goals and answer our experimental questions. The second part of the assignment is to narrow your focus to the experimental design of the first part of the project, our linkage analysis. You will explain in a narrative how the crosses you will set up are designed to answer the question, on which chromosome is the gene responsible for the aberrant phenotype observed in our mutant worms? In addition to the explanation, you will turn in a filled out template that shows your hypothesis about the outcome of the crosses you will set up in this part of our investigation.

Part 1: Summary of our Series 2 Classical (Forward Genetics) Investigation in C. elegans

Please construct a complete yet concise statement of the overall topic of investigation in Series 2 and the experimental question & goals of this classical (forward) genetics approach to understanding the structural and functional relationship between a gene and the product it encodes.

Part 2 Linkage Analysis :

Using the templates provided at Media:Series 2 Linkage Templates 5.pptx, construct a diagram of crosses that you will set up in the Linkage Testing part of Series 2. In the template, make sure to highlight the progeny that will be used to continue each step of the process of linkage testing. Include the expected results (genotype and phenotype) for both the outcross and self-fertilization progeny. Compose a narrative description that summarizes the rationale for Linkage Testing as an important part of your overall Series 2 goals. Explain what you are looking for in the results of these crosses that will allow you to determine the linkage group to which your mutated gene of interest belongs.

A Grading Rubric is found below.

Grading Rubrics

Assignment Part 1– 10 points
Series 2 summary of project

At or Above Standard Below Standard Possible
Points
Points

Earned

Topic
& Experimental Question
Constructed a clear, concise, and accurate description of the overall topic, the experimental question(s) and goals of the Series 2 investigation Topic or experimental question(s)/goals missing, inaccurate, partially accurate; contains too much tangential information or is too simplified 2 __/2
Outline
of Experimental Approach in Series 2: Classical or Forward Genetics
Constructed a complete, clear, concise, and accurate outline or flow diagram of ALL parts of Series2 including: test for true breeding, linkage testing to determine which chromosome the gene responsible for the mutation is found, mapping the mutation on a chromosome, complementation testing to determine if new mutation or previously characterized gene and mutation, DNA sequencing analysis & bioinformatics work to find structurally significant parts of the gene and functionally significant parts of the gene product, and to broaden the context and significance of the findings but a search for find homologs/orthologs. Outline/diagram is missing elements, is inaccurate or partially inaccurate; contains too much tangential information or is too simplified. 4 __/4
Narrative summary of project
Constructed a clear, concise, accurate, and logically developed description of the experimental approach to locating and characterizing the gene and gene mutation responsible for a phenotypic alteration from wild type Experimental analysis missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, isn't logically developed 4 __/4
Total 10 __/10


Assignment Part 2 (5 Points)

Grading Rubric

Linkage Analysis

At or Above Standard Below Standard Possible
Points
Points

Earned

Diagram of Crosses All possible cross outcomes diagrammed accurately, completely, clearly, and concisely. One or more possible crosses missing; one or more is inaccurate, information omitted , or did not include accurate predicted ratios of progeny for genotype and phenotype. 2.5 __/2.5
Narrative description
of rational for crosses
Constructed a clear, concise, and accurate description of the rational for setting up the crosses described Rational for crosses missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, is unclear 0.5 __/0.5
Narrative description
of Interpretation of Crosses
Constructed a clear, concise, and accurate description of how you will use the crosses to determine the linkage group to which your mutated gene belongs. Interpretation of crosses missing, inaccurate, partially accurate, or unclear; contains too much tangential information or is too simplified. 2 __/2
Total 5 __/5



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