BISC219/F13: Assignment Series2 Outline Summary

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(Part 2 Linkage Analysis :)
(Grading Rubric)
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| All possible cross outcomes diagrammed accurately, completely, clearly, and concisely.
| All possible cross outcomes diagrammed accurately, completely, clearly, and concisely.
| One or more possible crosses missing; one or more is inaccurate,  information omitted , or did not include accurate predicted ratios of progeny for genotype and phenotype.
| One or more possible crosses missing; one or more is inaccurate,  information omitted , or did not include accurate predicted ratios of progeny for genotype and phenotype.
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| 3.5
+
| 7
-
| __/3.5
+
| __/7
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|-
+
-
! Narrative description <BR>of rationale and interpretation of crosses
+
-
| Constructed a clear, concise, and accurate description of the rational for setting up the crosses described.Constructed a clear, concise, and accurate description of how you will use the crosses to determine the linkage group to which your mutated gene belongs.
+
-
| Rational for crosses missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, is unclear. Interpretation of crosses missing, inaccurate, partially accurate, or unclear; contains too much tangential information or is too simplified.
+
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| 3.5
+
-
| __/3.5
+
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|-
! Total  
! Total  

Revision as of 10:39, 23 August 2013

Contents

Assignment: Series 2 Summary/ Diagram & Explain Linkage Analysis

Your assignment due at the beginning of Lab 3 is in two parts. Part 1 is to summarize concisely the Series 2 project in classical (forward genetics). The Background information at Description of the Project in Series 2 should be helpful but remember to summarize this extensive explanation and use your own words! The goal of this part of the assignment is for you to have a complete and clear understanding of the broad topic, our investigative goals, and the experimental approach that we will use to accomplish these goals and answer our experimental questions. The second part of the assignment is to narrow your focus to the experimental design of the first part of the project, our linkage analysis. You will explain in a narrative how the crosses you will set up are designed to answer the question, on which chromosome is the gene responsible for the aberrant phenotype observed in our mutant worms? In addition to the explanation, you will turn in a filled out template that shows your hypothesis about the outcome of the crosses you will set up in this part of our investigation.

Part 1: Summary of our Series 2 Classical (Forward Genetics) Investigation in C. elegans

Please construct a complete yet concise statement of the overall topic of investigation in Series 2 and the experimental question & goals of this classical (forward) genetics approach to understanding the structural and functional relationship between a gene and the product it encodes.

Part 2 Linkage Analysis :

Using the templates provided at Media:Series 2 Linkage Templates 5.pptx, construct a diagram of crosses that you will set up in the Linkage Testing part of Series 2. In the template, make sure to highlight the progeny that will be used to continue each step of the process of linkage testing. Include the expected results (genotype and phenotype) for both the outcross and self-fertilization progeny.

A Grading Rubric is found below.

Grading Rubric

Series 2 summary of project/ Linkage Analysis

At or Above Standard Below Standard Possible
Points
Points

Earned

Topic
& Experimental Question
Constructed a clear, concise, and accurate description of the overall topic, the experimental question(s) and goals of the Series 2 investigation Topic or experimental question(s)/goals missing, inaccurate, partially accurate; contains too much tangential information or is too simplified 1 __/1
Narrative summary of project
Constructed a clear, concise, accurate, and logically developed description of the experimental approach to locating and characterizing the gene and gene mutation responsible for a phenotypic alteration from wild type Experimental analysis missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, isn't logically developed 2 __/2
Diagram of Crosses All possible cross outcomes diagrammed accurately, completely, clearly, and concisely. One or more possible crosses missing; one or more is inaccurate, information omitted , or did not include accurate predicted ratios of progeny for genotype and phenotype. 7 __/7
Total 10 __/10



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