BISC219/F13:RNA interference

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Revision as of 12:09, 20 August 2013

Wellesley College BISC 219 Genetics Fall 2013

These labs were modified from the work of with the Silencing Genomes project of the Dolan DNA Learning Center in Cold Spring Harbour, New York providing some of the worm strains.

Background Information on Project 3: Investigating Gene Function & Regulation Using RNAi
Media Recipes
Lab 6: Obtain CL2070 worms and propagate in your group
Lab 7: Examination of CL2070 hsp-16.2::GFP worms
Lab 8: Creating the feeding strain of bacteria for RNAi
Lab 9: Induction of feeding strain to produce dsRNA and feeding worms
Lab 10: Phenotypic analysis of treated vs untreated worms
Lab 11:



Schedule of Experiments

Lab # Dates Activity Outside lab time
6 10/16-10/22 Begin a culture of CL2070 worms
7 10/22 - 10/28 Examine the CL2070 strain of worms
8 11/4 - 11/8 Transform hsf-1 feeding RNAi plasmid into the HT115DE3 strain The day after lab: check your transformed bacteria for colony growth

The night before next lab: set up an overnight culture of your colony of interest

9 11/11 - 11/15 Induction of dsRNA production, seeding of RNAi plates Add different strains of worms to feeding plates at appropriate time
10 11/18 - 11/22 Students score N2, rrf-3 worms for phenotypes and examine the CL2070 worms under the fluorescent scope
11 11/26 - 11/30 Writing Workshop