Lab 10: Series 3 Investigation of Gene Regulation Using RNAi--
Preparation of the Na+ gradient in the Chemotaxis Assay media
The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates.
- 10 ul of 2.5M NaCl is added to the Na dot you drew on your plate. Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot. The Cl2 is not in a gradient due to the addition of MgCl2 to the media during initial preparation.
- 10 ul of sterile water is added to the W dot you drew to serve as a negative control.