BISC219/F12: RNAi Lab 10: Difference between revisions
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==Lab 10: Series 3 Investigation of Gene Regulation Using RNAi-- == | ==Lab 10: Series 3 Investigation of Gene Regulation Using RNAi-- == | ||
'''Preparation of the Na+ gradient in the Chemotaxis Assay media'''<br> | |||
The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates. <br> | |||
#10 ul of 2.5M NaCl is added to the Na dot you drew on your plate. Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot. The Cl<sub>2</sub> is not in a gradient due to the addition of MgCl<sub>2</sub> to the media during initial preparation. | |||
#10 ul of sterile water is added to the W dot you drew to serve as a negative control. | |||
Revision as of 08:33, 5 November 2012
Lab 10: Series 3 Investigation of Gene Regulation Using RNAi--
Preparation of the Na+ gradient in the Chemotaxis Assay media
The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates.
- 10 ul of 2.5M NaCl is added to the Na dot you drew on your plate. Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot. The Cl2 is not in a gradient due to the addition of MgCl2 to the media during initial preparation.
- 10 ul of sterile water is added to the W dot you drew to serve as a negative control.
