BISC219/F12: RNAi Lab 10
From OpenWetWare
(Difference between revisions)
(→Lab 10: Series 3 Investigation of Gene Regulation Using RNAi--) |
|||
| Line 3: | Line 3: | ||
==Lab 10: Series 3 Investigation of Gene Regulation Using RNAi-- == | ==Lab 10: Series 3 Investigation of Gene Regulation Using RNAi-- == | ||
| - | + | '''Preparation of the Na+ gradient in the Chemotaxis Assay media'''<br> | |
| + | The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates. <br> | ||
| + | #10 ul of 2.5M NaCl is added to the Na dot you drew on your plate. Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot. The Cl<sub>2</sub> is not in a gradient due to the addition of MgCl<sub>2</sub> to the media during initial preparation. | ||
| + | #10 ul of sterile water is added to the W dot you drew to serve as a negative control. | ||
| + | |||
Revision as of 11:33, 5 November 2012
Lab 10: Series 3 Investigation of Gene Regulation Using RNAi--
Preparation of the Na+ gradient in the Chemotaxis Assay media
The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates.
- 10 ul of 2.5M NaCl is added to the Na dot you drew on your plate. Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot. The Cl2 is not in a gradient due to the addition of MgCl2 to the media during initial preparation.
- 10 ul of sterile water is added to the W dot you drew to serve as a negative control.
