BISC219/F12: RNAi Lab 10

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(Lab 10: Series 3 Investigation of Gene Regulation Using RNAi--)
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==Lab 10: Series 3 Investigation of Gene Regulation Using RNAi-- ==
==Lab 10: Series 3 Investigation of Gene Regulation Using RNAi-- ==
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'''Preparation of the Na+ gradient in the Chemotaxis Assay media'''<br>
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The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates.  <br>
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#10 ul of 2.5M NaCl is added to the Na dot you drew on your plate.  Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot.  The Cl<sub>2</sub> is not in a gradient due to the addition of MgCl<sub>2</sub> to the media during initial preparation.
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#10 ul of sterile water is added to the W dot you drew to serve as a negative control.
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Revision as of 11:33, 5 November 2012

Lab 10: Series 3 Investigation of Gene Regulation Using RNAi--

Preparation of the Na+ gradient in the Chemotaxis Assay media
The day before lab your instructor will add the chemicals needed to prepare the chemotaxis assay plates.

  1. 10 ul of 2.5M NaCl is added to the Na dot you drew on your plate. Once the NaCl is absorbed the Na+ ions will disperse in a gradient away from the dot. The Cl2 is not in a gradient due to the addition of MgCl2 to the media during initial preparation.
  2. 10 ul of sterile water is added to the W dot you drew to serve as a negative control.


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