BISC219/F12:RNA interference

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Wellesley College BISC 219 Genetics

These labs were developed with the help of the Silencing Genomes project of the Dolan DNA Learning Center.

RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms
Lab 11: Individual Writing Conferences


Schedule of Experiments

Lab # Dates Activity Outside lab time
7 10/23 - 10/29 The night before next lab:
8 10/30 - 11/5 The day after lab:

The night before next lab:

9 11/7 - 11/13 4 days later:
10 11/14 - 11/20
11 11/26 - 11/303
12 12/ - 12/7