BISC219/F12:RNA interference - Revision history

Tucker Crum at 01:04, 19 November 2012

Tucker Crum — Mon, 19 Nov 2012 01:04:05 GMT

←Older revision		Revision as of 01:04, 19 November 2012
Line 9:		Line 9:
	[[BISC219/F12: RNAi Lab 9 \| Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br>		[[BISC219/F12: RNAi Lab 9 \| Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br>
	[[BISC219/F12: RNAi Lab 10 \| Lab 10: Phenotypic analysis of treated vs untreated worms]]<br>		[[BISC219/F12: RNAi Lab 10 \| Lab 10: Phenotypic analysis of treated vs untreated worms]]<br>
-	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ~~Writing~~ Workshop]]<br>	+	[[BISC219/F12: RNAi Lab 11 \| Lab 11: Data Analysis Workshop]]<br>
-	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ~~Writing~~ Conferences]]<br>	+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: Optional Conferences]]<br>
	<br>		<br>

Melissa Beers at 15:25, 10 October 2012

Melissa Beers — Wed, 10 Oct 2012 15:25:03 GMT

←Older revision		Revision as of 15:25, 10 October 2012
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	<div style="padding: 10px; width: 720px; border: 5px solid #7E354D;">		<div style="padding: 10px; width: 720px; border: 5px solid #7E354D;">

-	These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with ~~help from~~ the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.<br><br>	+	These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York. providing some of the worm strains.<br><br>
	[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Function & Regulation Using RNAi]] <br>		[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Function & Regulation Using RNAi]] <br>
	[[BISC219/F12: Media Recipes \| Media Recipes]]<br>		[[BISC219/F12: Media Recipes \| Media Recipes]]<br>

Tucker Crum at 18:05, 22 August 2012

Tucker Crum — Wed, 22 Aug 2012 18:05:21 GMT

←Older revision		Revision as of 18:05, 22 August 2012
Line 3:		Line 3:

	These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.<br><br>		These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.<br><br>
-	[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br>	+	[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Function & Regulation Using RNAi]] <br>
	[[BISC219/F12: Media Recipes \| Media Recipes]]<br>		[[BISC219/F12: Media Recipes \| Media Recipes]]<br>
	[[BISC219/F12: RNAi Lab 7 \| Lab 7: Identifying a bacterial colony containing our plasmid of interest ]]<br>		[[BISC219/F12: RNAi Lab 7 \| Lab 7: Identifying a bacterial colony containing our plasmid of interest ]]<br>

Melissa Beers at 01:40, 22 August 2012

Melissa Beers — Wed, 22 Aug 2012 01:40:00 GMT

←Older revision		Revision as of 01:40, 22 August 2012
Line 5:		Line 5:
	[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br>		[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br>
	[[BISC219/F12: Media Recipes \| Media Recipes]]<br>		[[BISC219/F12: Media Recipes \| Media Recipes]]<br>
-	[[BISC219/F12: RNAi Lab 7 \| Lab 7: ]]<br>	+	[[BISC219/F12: RNAi Lab 7 \| Lab 7: Identifying a bacterial colony containing our plasmid of interest ]]<br>
-	[[BISC219/F12: RNAi Lab 8 \| Lab 8: ]]<br>	+	[[BISC219/F12: RNAi Lab 8 \| Lab 8: Creating the feeding strain of bacteria for RNAi]]<br>
-	[[BISC219/F12: RNAi Lab 9 \| Lab 9: ]]<br>	+	[[BISC219/F12: RNAi Lab 9 \| Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br>
-	[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>	+	[[BISC219/F12: RNAi Lab 10 \| Lab 10: Phenotypic analysis of treated vs untreated worms]]<br>
-	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br>	+	[[BISC219/F12: RNAi Lab 11 \| Lab 11: Writing Workshop]]<br>
-	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ]]<br>	+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: Writing Conferences]]<br>
	<br>		<br>

Melissa Beers: /* Schedule of Experiments */

Melissa Beers — Wed, 22 Aug 2012 01:34:29 GMT

Schedule of Experiments

←Older revision		Revision as of 01:34, 22 August 2012
Line 22:		Line 22:
	!7		!7
	\|10/23 - 10/29		\|10/23 - 10/29
-	\| Picking a colony of bacteria containing the RNAi plasmid	+	\| Picking a colony of bacteria containing the RNAi plasmid<br>
		+	PCR to confirm presence of gene of interest
	\|'''The night before next lab:''' set up an overnight culture of your colony of interest<br>		\|'''The night before next lab:''' set up an overnight culture of your colony of interest<br>
-
	\|-		\|-
	!8		!8
	\|10/30 - 11/5		\|10/30 - 11/5
-	\| ~~PCR to confirm presence of gene of interest, miniprep~~ of plasmid and transformation into feeding bacteria strain	+	\| Miniprep of plasmid and transformation into feeding bacteria strain
	\|'''The day after lab:''' check your transformed bacteria for colony growth<br>		\|'''The day after lab:''' check your transformed bacteria for colony growth<br>
	'''The night before next lab:''' set up an overnight culture of your colony of interest<br>		'''The night before next lab:''' set up an overnight culture of your colony of interest<br>

Melissa Beers: /* Schedule of Experiments */

Melissa Beers — Tue, 21 Aug 2012 03:10:09 GMT

Schedule of Experiments

←Older revision		Revision as of 03:10, 21 August 2012
Line 22:		Line 22:
	!7		!7
	\|10/23 - 10/29		\|10/23 - 10/29
-	\|	+	\| Picking a colony of bacteria containing the RNAi plasmid
-	\|'''The night before next lab:'''<br>	+	\|'''The night before next lab:''' set up an overnight culture of your colony of interest<br>

	\|-		\|-
	!8		!8
	\|10/30 - 11/5		\|10/30 - 11/5
-	\|	+	\| PCR to confirm presence of gene of interest, miniprep of plasmid and transformation into feeding bacteria strain
-	\|'''The day after lab:'''<br>	+	\|'''The day after lab:''' check your transformed bacteria for colony growth<br>
-	'''The night before next lab:'''<br>	+	'''The night before next lab:''' set up an overnight culture of your colony of interest<br>
	\|-		\|-
	!9		!9
	\|11/7 - 11/13		\|11/7 - 11/13
		+	\| Induction of dsRNA production, seeding of RNAi plates and addition of worms
	\|		\|
-	~~\|'''4 days later:'''<br>~~
	\|-		\|-
	!10		!10
	\|11/14 - 11/20		\|11/14 - 11/20
-	\|	+	\| Chemotaxis Assay
	\|		\|
	\|-		\|-
	!11		!11
-	\|11/26 - 11/~~303~~	+	\|11/26 - 11/30
-	\|	+	\| Writing Workshop
	\|		\|
	\|-		\|-
	!12		!12
-	\|12/ - 12/7	+	\|12/3 - 12/7
-	\|	+	\| Writing Conferences
	\|		\|
	\|-		\|-

Tucker Crum at 20:06, 20 August 2012

Tucker Crum — Mon, 20 Aug 2012 20:06:32 GMT

←Older revision		Revision as of 20:06, 20 August 2012
Line 10:		Line 10:
	[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>		[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>
	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br>		[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br>
-	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ]]<br	+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ]]<br>
	<br>		<br>

Tucker Crum at 20:06, 20 August 2012

Tucker Crum — Mon, 20 Aug 2012 20:06:21 GMT

←Older revision		Revision as of 20:06, 20 August 2012
Line 9:		Line 9:
	[[BISC219/F12: RNAi Lab 9 \| Lab 9: ]]<br>		[[BISC219/F12: RNAi Lab 9 \| Lab 9: ]]<br>
	[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>		[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>
-	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br><br>	+	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br>
		+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ]]<br
		+	<br>

Tucker Crum at 20:05, 20 August 2012

Tucker Crum — Mon, 20 Aug 2012 20:05:44 GMT

←Older revision		Revision as of 20:05, 20 August 2012
Line 3:		Line 3:

	These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.<br><br>		These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.<br><br>
-	[[BISC219/F12: RNAi General Information\| ~~RNAi General~~ Information]] <br>	+	[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br>
	[[BISC219/F12: Media Recipes \| Media Recipes]]<br>		[[BISC219/F12: Media Recipes \| Media Recipes]]<br>
	[[BISC219/F12: RNAi Lab 7 \| Lab 7: ]]<br>		[[BISC219/F12: RNAi Lab 7 \| Lab 7: ]]<br>

Tucker Crum at 20:04, 20 August 2012

Tucker Crum — Mon, 20 Aug 2012 20:04:56 GMT

←Older revision		Revision as of 20:04, 20 August 2012
Line 2:		Line 2:
	<div style="padding: 10px; width: 720px; border: 5px solid #7E354D;">		<div style="padding: 10px; width: 720px; border: 5px solid #7E354D;">

-	These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the '''Silencing Genomes''' project of the Dolan DNA Learning Center.<br><br>	+	These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the '''Silencing Genomes''' project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.<br><br>
	[[BISC219/F12: RNAi General Information\| RNAi General Information]] <br>		[[BISC219/F12: RNAi General Information\| RNAi General Information]] <br>
	[[BISC219/F12: Media Recipes \| Media Recipes]]<br>		[[BISC219/F12: Media Recipes \| Media Recipes]]<br>

←Older revision		Revision as of 01:04, 19 November 2012
Line 9:		Line 9:
	[[BISC219/F12: RNAi Lab 9 \| Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br>		[[BISC219/F12: RNAi Lab 9 \| Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br>
	[[BISC219/F12: RNAi Lab 10 \| Lab 10: Phenotypic analysis of treated vs untreated worms]]<br>		[[BISC219/F12: RNAi Lab 10 \| Lab 10: Phenotypic analysis of treated vs untreated worms]]<br>
-	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ~~Writing~~ Workshop]]<br>	+	[[BISC219/F12: RNAi Lab 11 \| Lab 11: Data Analysis Workshop]]<br>
-	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ~~Writing~~ Conferences]]<br>	+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: Optional Conferences]]<br>
	<br>		<br>

←Older revision		Revision as of 01:40, 22 August 2012
Line 5:		Line 5:
	[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br>		[[BISC219/F12: RNAi General Information\| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br>
	[[BISC219/F12: Media Recipes \| Media Recipes]]<br>		[[BISC219/F12: Media Recipes \| Media Recipes]]<br>
-	[[BISC219/F12: RNAi Lab 7 \| Lab 7: ]]<br>	+	[[BISC219/F12: RNAi Lab 7 \| Lab 7: Identifying a bacterial colony containing our plasmid of interest ]]<br>
-	[[BISC219/F12: RNAi Lab 8 \| Lab 8: ]]<br>	+	[[BISC219/F12: RNAi Lab 8 \| Lab 8: Creating the feeding strain of bacteria for RNAi]]<br>
-	[[BISC219/F12: RNAi Lab 9 \| Lab 9: ]]<br>	+	[[BISC219/F12: RNAi Lab 9 \| Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br>
-	[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>	+	[[BISC219/F12: RNAi Lab 10 \| Lab 10: Phenotypic analysis of treated vs untreated worms]]<br>
-	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br>	+	[[BISC219/F12: RNAi Lab 11 \| Lab 11: Writing Workshop]]<br>
-	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ]]<br>	+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: Writing Conferences]]<br>
	<br>		<br>

←Older revision		Revision as of 20:06, 20 August 2012
Line 9:		Line 9:
	[[BISC219/F12: RNAi Lab 9 \| Lab 9: ]]<br>		[[BISC219/F12: RNAi Lab 9 \| Lab 9: ]]<br>
	[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>		[[BISC219/F12: RNAi Lab 10 \| Lab 10: ]]<br>
-	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br><br>	+	[[BISC219/F12: RNAi Lab 11 \| Lab 11: ]]<br>
		+	[[BISC219/F12: RNAi Lab 12 \| Lab 12: ]]<br
		+	<br>