BISC219/F12:RNA interference: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
||
Line 21: | Line 21: | ||
|- | |- | ||
!7 | !7 | ||
|10/ | |10/23 - 10/29 | ||
| | | | ||
|'''The night before next lab:'''<br> | |'''The night before next lab:'''<br> | ||
Line 27: | Line 27: | ||
|- | |- | ||
!8 | !8 | ||
| | |10/30 - 11/5 | ||
| | | | ||
|'''The day after lab:'''<br> | |'''The day after lab:'''<br> | ||
Line 33: | Line 33: | ||
|- | |- | ||
!9 | !9 | ||
|11/ | |11/7 - 11/13 | ||
| | | | ||
|'''4 days later:'''<br> | |'''4 days later:'''<br> | ||
|- | |- | ||
!10 | !10 | ||
|11/ | |11/14 - 11/20 | ||
| | | | ||
| | | | ||
|- | |- | ||
!11 | !11 | ||
|11/ | |11/26 - 11/303 | ||
| | | | ||
| | | | ||
|- | |- | ||
!12 | !12 | ||
|12/ - 12/ | |12/ - 12/7 | ||
| | | | ||
| | | | ||
|- | |- |
Revision as of 12:22, 20 August 2012
These labs were developed with the help of the Silencing Genomes project of the Dolan DNA Learning Center.
RNAi General Information
Media Recipes
Lab 5: Picking your gene to RNAi
Lab 6: Cloning your gene of interest
Lab 7: Picking your transformant
Lab 8: Plasmid purification and transformation
Lab 9: Induction of bacteria for RNAi
Lab 10: Scoring your worms
Lab 11: Individual Writing Conferences
Schedule of Experiments
Lab # | Dates | Activity | Outside lab time |
---|---|---|---|
7 | 10/23 - 10/29 | The night before next lab: | |
8 | 10/30 - 11/5 | The day after lab: The night before next lab: | |
9 | 11/7 - 11/13 | 4 days later: | |
10 | 11/14 - 11/20 | ||
11 | 11/26 - 11/303 | ||
12 | 12/ - 12/7 |