BISC209/S13: Assignment 209 Lab8

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Wellesley College-BISC 209 Microbiology -Spring 2013


Lab 8: Isolate structure and functional capacity table

Graded Assignment: Make separate figures and text for the antibiotic assay (if any of your site teammates indicated the presence of an antibiotic producer) and interactions assays. Use the Google doc table of the tests performed on the isolates from your soil community (include your teammates' isolates from the same sampling site for this assignment) to make a Table and write the results narrative for this table. You should include in the table: Gram stain, enrichment/selection medium, description of the colony morphology, description of the individual bacteria shape (cocci, bacilli, spirilla) and any useful descriptors ie., large, small, bullet shaped, in chains, etc., evidence of endospores (either endospore stain positive or visualization of empty areas in vegetative cells on Gram stain), & motility. Include metabolic tests such as nitrate reduction and mannitol fermentation. Leave out the interaction assay and the antibiotic producers test (if your site had a positive) in this table and make separate results figures from photographs of those plates and discuss those findings in separate sections in results. Work with ALL of your sampling sites' interaction assays on this results section and a photo of any positive antibiotic producers from your sampling site for that section of this draft results. If your site had no positive antibiotic producers, you can include this test and its negative results in your table with out a photo or separate section.