BISC209/S13:Assignments

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Wellesley College-BISC 209 Microbiology -Spring 2013

Graded Lab Assignments

Lab Assigned Lab Due Assignment
description
Point Value
(Total 200)
1 2 Lab 1 Assignment Overview with References of culture techniques and media. Detailed version: Assignment: Enrichment/Selection/Differentiation of Culturable Bacteria. 5
2 3 Lab 2 Assignment Explain The Great Plate Count Anomaly. Detailed version: Assignment: Introduction1. 5
3 4 Lab 3 Assignment Enumeration data Results: Design and Analysis. Detailed version: Lab 3 Assignment: Colony Count vs. Direct Count Enumeration 5
4 5 Lab 4 Assignment Community level diversity and behavior Results: Design and Analysis Detailed version: BISC209/S13: Assignment_209_BIOLOG 10
5 6 Lab 5 Assignment M&M: Partial Materials and Methods section. Detailed version: Materials & Methods 10
6 7 Lab 6 Assignment PCR and Sanger sequence Theory and Bacterial ID techniques theory. Detailed version: Assignment: Theory Summary 10
7 8 Lab 7 Assignment Discussion Outline, Annotated Bibliography (10 points) and Graphical abstract (5 points): Detailed version:: Assignment: Annotated Bibliography/Graphical Abstract 15
8 9 Lab 8 Assignment Isolate table of the tests performed: Design and Analysis. Detailed version: [[BISC209/S13: Assignment_209_Lab8}}

Construct a on the isolates from your soil community (your isolates and your teammates from the same sampling site) and write a complete results narrative. You should include in the table: Gram stain, description of the colony morphology, description of the individual bacteria's structural characteristics including shape & arrangement (cocci, rods, with descriptors ie., large, small, bullet shaped, in chains, etc.), evidence of spores (either endospore stain positive or visualization of empty areas in vegetative cells on Gram stain), motility, etc. Include test results that you will use as evidence for metabolic diversity or community behavior, ie. nitrogen cycling capabilities, fermentation of mannitol, etc. Make separate figures from photographs of the interactions assays for your soil sample and another from a photo of a positive antibiotic production assay (if your soil sample showed such a positive) and analyze those data in your narrative. Post your table to the Data folder for each soil sampling site so your classmates can access and use this information for the other bacterial members of each soil community.

10
9 10 Results Table and analysis of isolate identification data 5
10 10 Lab Practical 40
May 7 or 8 Final Paper: Information found at: Assignment: Final Paper 75
May 7 or 8 Lab notebook 5
Other CLEAN-UP points 5


Assignments

Lab 1 Assignment: Assignment: Enrichment/Selection/Differentiation of Culturable Bacteria
Lab 2 Assignment: Assignment: Introduction
Lab 3 Assignment: Lab 3 Assignment: Colony Count vs. Direct Count Enumeration
Lab 4 Assignment: BISC209/S13: Assignment_209_BIOLOG
Lab 5 Assignment: Assignment: Materials & Methods
Lab 6 Assignment: Assignment: Theory Summary
Lab 7 Assignment: Assignment: Annotated Bibliography/Graphical Abstract
Lab 10 Assignment: Assignment: Final Paper