BISC209/S13:Assignments: Difference between revisions

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| [[BISC209/S13: Lab3 | Lab 3 Assignment]] '''Table Design and Analysis.'''  Detailed version: [[BISC209/S13:Assignment_209_Lab3 | Lab 3 Assignment: Colony Count vs. Direct Count Enumeration]]
| [[BISC209/S13: Lab3 | Lab 3 Assignment]] '''Enumeration data Results: Design and Analysis.'''  Detailed version: [[BISC209/S13:Assignment_209_Lab3 | Lab 3 Assignment: Colony Count vs. Direct Count Enumeration]]
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| Analyze your community level diversity and behavior data including the prevalence of microbial starch & cellulose digesters and phosphate solubilizers in the soil community and the carbon source utilization patterns and CMD measurement. Write a results section with figures/tables and narrative. Detailed directions and useful references for this assignment can be found at  Lab 4 Assignment:  [[BISC209/S13: Assignment_209_BIOLOG]]
|[[BISC209/S13: Lab4 | Lab 4 Assignment]]  ''' Community level diversity and behavior Results:  Design and Analysis'''    Detailed version:  [[BISC209/S13: Assignment_209_BIOLOG]]
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| '''M&M''': Compose a draft of your Materials and Methods section of your final paper with the following three general sections:<br> 1)Community level physiological testing: carbon source profiling, exoenzymes profiling; <BR> 2) Identification of bacteria by 16S rRNA gene sequencing;<br> 3) Selection and isolation of soil community bacteria to pure culture.<BR> Detailed directions can be found at Lab 5 Assignment: [[BISC209/S13: Assignment_209_Lab5 | Materials & Methods]]
|[[BISC209/S13: Lab5 | Lab 5 Assignment]]  '''M&M''': Partial Materials and Methods section. Detailed version:   [[BISC209/S13: Assignment_209_Lab5 | Materials & Methods]]
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| Write a brief summary of the theory behind the following techniques that we used to identify our bacterial species by molecular tools: polymerase chain amplification of the 16srRNA gene and DNA sequencing by the newer fluorescent-labeled ddNPTs chain -termination (Sanger) method. Detailed irections can be found at: Lab 6 Assignment:  [[BISC209/S13: Assignment_209_Lab6 | Assignment: Theory Summary ]]
| [[BISC209/S13: Lab6 | Lab 6 Assignment]]  '''PCR, Sanger sequence, and Bacterial ID techniques theory. Detailed version:  [[BISC209/S13: Assignment_209_Lab6 | Assignment: Theory Summary ]]
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| '''Discussion Outline''', '''Annotated Bibliography''' of appropriate references for the discussion section of your paper (5 points)<BR>'''Graphical abstract''' draft (5 points): See models in research reports found in recent issues of the journal ''Cell''. A description and examples of Graphical Abstracts can be found at http://www.elsevier.com/wps/find/authorsview.authors/graphicalabstracts [http://www.elsevier.com/wps/find/authorsview.authors/graphicalabstracts]. Detailed directions on this assignment can be found at: [[BISC209/S13: Assignment_209_Lab7 | Assignment: Annotated Bibliography/Graphical Abstract]]
| [[BISC209/S13: Lab7 | Lab 7 Assignment]] '''Discussion Outline''', '''Annotated Bibliography''' (10 points) and '''Graphical abstract''' (5 points): Detailed version:: [[BISC209/S13: Assignment_209_Lab7 | Assignment: Annotated Bibliography/Graphical Abstract]]
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|'''Write a draft results section of your work on your isolates.''' Construct a table of the tests performed on the isolates from your soil community (your isolates and your teammates from the same sampling site) and write a complete results narrative. You should include in the table: Gram stain, description of the colony morphology, description of the individual bacteria's structural characteristics including shape & arrangement (cocci, rods, with descriptors ie., large, small, bullet shaped, in chains, etc.), evidence of spores (either endospore stain positive or visualization of empty areas in vegetative cells on Gram stain), motility, etc. Include test results that you will use as evidence for metabolic diversity or community behavior, ie. nitrogen cycling capabilities, fermentation of mannitol, etc. Make separate figures from photographs of the interactions assays for your soil sample and another from a photo of a positive antibiotic production assay (if your soil sample showed such a positive) and analyze those data in your narrative. Post your table to the Data folder for each soil sampling site so your classmates can access and use this information for the other bacterial members of each soil community.
|[[BISC209/S13: Lab8 | Lab 8 Assignment]]  '''Isolate table of tests performed and evidence of co-operation and competition: Design and Analysis.'''  Detailed version: [[BISC209/S13: Assignment_209_Lab8 | Assignment Isolate tables and figures]]
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| '''Results Table and analysis of isolate identification data'''  
|[[BISC209/S13: Lab9 | Lab 9 Assignment]]  '''Study for the Lab Practical.'''   We hope you are working on the final revisions of your paper, pass in a draft next lab for 5 points.  Detailed version:  [[BISC209/S13: Assignment 209 Lab9 | Lab Practical tips]]  and [[BISC209/S13: Assignment 209 Lab10 | Final Paper tips]] <BR><BR>
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| '''Lab Practical
|[[BISC209/S13: Lab10 | Lab 10 Assignment]]  '''Lab Practical'''  & [[BISC209/S13: Assignment 209 Lab10 | Complete Rough Draft of Final Paper]]
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| '''May 7 or 8'''
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| '''Final Paper''': Information found at: [[BISC209/S13: Assignment_209_Lab10 | Assignment: Final Paper]]
| '''Final Paper''': Information found at: [[BISC209/S13: Assignment_209_Lab10 | Assignment: Final Paper]] and [[BISC209/S13:Resources | Resources]]
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| '''May 7 or 8'''
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| '''Lab notebook'''
| '''Lab notebook''' Information found at:  [[BISC209/S13:Guidelines for maintaining your lab notebook | Lab Notebook Guidelines]]
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==Assignments==
 
Lab 1 Assignment: [[BISC209/S13: Assignment_209_Lab1 | Assignment: Enrichment/Selection/Differentiation of Culturable Bacteria]]<BR>
Lab 2 Assignment:  [[BISC209/S13: Assignment_209_Lab2 | Assignment: Introduction]]<BR>
Lab 3 Assignment: [[BISC209/S13:Assignment_209_Lab3 | Lab 3 Assignment: Colony Count vs. Direct Count Enumeration]]<BR>
Lab 4 Assignment:  [[BISC209/S13: Assignment_209_BIOLOG]]<BR>
Lab 5 Assignment:  [[BISC209/S13: Assignment_209_Lab5 | Assignment: Materials & Methods]]<BR>
Lab 6 Assignment:  [[BISC209/S13: Assignment_209_Lab6 | Assignment: Theory Summary ]]<BR>
Lab 7 Assignment:  [[BISC209/S13: Assignment_209_Lab7 | Assignment: Annotated Bibliography/Graphical Abstract]]<BR>
Lab 10 Assignment: [[BISC209/S13: Assignment_209_Lab10 | Assignment: Final Paper]]<br>


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Latest revision as of 09:36, 30 January 2013

Wellesley College-BISC 209 Microbiology -Spring 2013

Graded Lab Assignments

Lab Assigned Lab Due Assignment
description		 Point Value
(Total 200)
1 2 Lab 1 Assignment Overview with References of culture techniques and media. Detailed version: Assignment: Enrichment/Selection/Differentiation of Culturable Bacteria. 5
2 3 Lab 2 Assignment Explain The Great Plate Count Anomaly. Detailed version: Assignment: Introduction1. 5
3 4 Lab 3 Assignment Enumeration data Results: Design and Analysis. Detailed version: Lab 3 Assignment: Colony Count vs. Direct Count Enumeration 5
4 5 Lab 4 Assignment Community level diversity and behavior Results: Design and Analysis Detailed version: BISC209/S13: Assignment_209_BIOLOG 10
5 6 Lab 5 Assignment M&M: Partial Materials and Methods section. Detailed version: Materials & Methods 10
6 7 Lab 6 Assignment PCR, Sanger sequence, and Bacterial ID techniques theory. Detailed version: Assignment: Theory Summary 10
7 8 Lab 7 Assignment Discussion Outline, Annotated Bibliography (10 points) and Graphical abstract (5 points): Detailed version:: Assignment: Annotated Bibliography/Graphical Abstract 15
8 9 Lab 8 Assignment Isolate table of tests performed and evidence of co-operation and competition: Design and Analysis. Detailed version: Assignment Isolate tables and figures 10
9 10 Lab 9 Assignment Study for the Lab Practical. We hope you are working on the final revisions of your paper, pass in a draft next lab for 5 points. Detailed version: Lab Practical tips and Final Paper tips

5
9 10 Lab 10 Assignment Lab Practical & Complete Rough Draft of Final Paper 40
11 May 7 or 8 Final Paper: Information found at: Assignment: Final Paper and Resources 75
11 May 7 or 8 Lab notebook Information found at: Lab Notebook Guidelines 5
Other CLEAN-UP points 5



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