BISC110/F12:Series2: Difference between revisions
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'''In this three week series of experiments you will learn:''' <br> | '''In this three week series of experiments you will learn:''' <br> | ||
#About SNPs and how they are analyzed. | #About SNPs and how they are analyzed. | ||
#How to isolate DNA from | #How to isolate DNA from bacterial cells. | ||
#How to use PCR to amplify a region of | #How to use PCR to amplify a region of the PTC gene which contains a SNP. | ||
#How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants. | #How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants. | ||
#How | #How genotype and phenotype at the PTC locus correlate. | ||
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html). | The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html). | ||
<br><br> | <br><br> | ||
Latest revision as of 11:10, 8 June 2012
Links to Labs in Series 2
Lab 6 Taster SNP 1
Lab 7: Taster SNP 2
Lab 8: Taster Data Analysis & Photosynthesis 1
Series 2- To Taste Or Not To Taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from bacterial cells.
- How to use PCR to amplify a region of the PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
