BIOPIC SUN:Inventory: Difference between revisions

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,,plasmid,Cat.No.,,,,,,
 
,109 -20,pet28a,69864-3,Novagen,,5369bp,,,require liuzhen's permit
{| border="1" cellpadding="11" cellspacing="0"
,109 -20,pet21b,69741-3,Novagen,,5443bp,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''序号'''
,109 -20,pbadmychisA,V440-01,Invitrogen��kindly provided by Jiwei Liu��,,4.1kb,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''存放位置'''
,109 -20,pacycduet-1,71147-3,Novagen��kindly provided by Jiwei Liu��,,4008bp,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''试剂名称'''
,-80,pcdfduet-1,71340-3,Novagen,,3781bp,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''货号'''
,109 -20,pkd3��chloramphenicol gene template��,,Kirill A. Datsenko PNAS 2000,,,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''公司'''
,109 -20,pkd4��kanamycin gene template ��,,Kirill A. Datsenko PNAS 2000,,,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''纯度'''
,109 -20,pkd13��kanamycin gene template ��,,Kirill A. Datsenko PNAS 2000,,,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''分子量'''
,109 -20,pkd46(lambda red recominase),,Kirill A. Datsenko PNAS 2000,,,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''日期'''
,109 -20,pcp20(eliminate the resistant gene),,Kirill A. Datsenko PNAS 2000,,,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''分子式'''
,109 -20,psnap,,NEB,,5802bp,,,require liuzhen's permit
| align="center" style="background:#f0f0f0;"|'''备注'''
,109 -20,pclipf,,NEB,,5862bp,,,require liuzhen's permit
|-
,109 -20,IrisFP,,provided by Haodong Chen,,,,,require liuzhen's permit
| ||||plasmid||Cat.No.||||||||||||
,109 -20,mEos2,,provided by Sunney's group  ,,,,,require liuzhen's permit
|-
,109 -20,mEos3.1,,provided by Pingyong Xu,,,,,require liuzhen's permit
| ||109 -20||pet28a||69864-3||Novagen||||5369bp||||||require liuzhen's permit
,109 -20,mEos3.2,,provided by Pingyong Xu,,,,,require liuzhen's permit
|-
,109 -20,Pdg1-s1(dronpa),AM-V0071,Amalgaam,,,,,require liuzhen's permit
| ||109 -20||pet21b||69741-3||Novagen||||5443bp||||||require liuzhen's permit
,109 -20,VAMPZ-ps-cfp2(K+),,provided by Tianyi yuan from Liangyi Chen's Lab,,,,,require liuzhen's permit
|-
,109 -20,syt7-pa-gfp(A+),,provided by Tianyi yuan from Liangyi Chen's Lab,,,,,require liuzhen's permit
| ||109 -20||pbadmychisA||V440-01||Invitrogen(kindly provided by Jiwei Liu)||||4.1kb||||||require liuzhen's permit
,109 -20,LOV2-Ja optogenetics ,,from Denise Montell JHCL ,,,,,require liuzhen's permit
|-
,109 -20,pUC19 Vector 1mg/ml,N3041S,NEB,,,,,xingdong
| ||109 -20||pacycduet-1||71147-3||Novagen(kindly provided by Jiwei Liu)||||4008bp||||||require liuzhen's permit
,109 -20,petduet,71146-3,Novagen,,,,,kongxinyu
|-
,,strain,,,,,,,
| ||-80||pcdfduet-1||71340-3||Novagen||||3781bp||||||require liuzhen's permit
,-80,bw25993,,provided by sunney'group,,,,,require liuzhen's permit
|-
,-80,bw25993+pkd46,,provided by sunney'group,,,,,require liuzhen's permit
| ||109 -20||pkd3(chloramphenicol gene template)||||Kirill A. Datsenko PNAS 2000||||||||||require liuzhen's permit
,-80,bl21(ai) ,,provided by sunney'group,,,,,require liuzhen's permit
|-
,-80,BL21(DE3) competent cell,,Transgene,,,,,
| ||109 -20||pkd4(kanamycin gene template )||||Kirill A. Datsenko PNAS 2000||||||||||require liuzhen's permit
,-80,trans5a competent cell,,Transgene,,,,,
|-
,-80,E.coli strain sx322 resistant to kanamycin ,,provided by sunney'group,,,,,require liuzhen's permit
| ||109 -20||pkd13(kanamycin gene template )||||Kirill A. Datsenko PNAS 2000||||||||||require liuzhen's permit
,-80,E.coli malI-tetR::eYFP resistant to kanamycin ,,provided by sunney'group,,,,,require liuzhen's permit
|-
,-80,"6tetO-cam@O2 of Lac,resistant to chloramphenicol ",,provided by sunney'group,,,,,require liuzhen's permit
| ||109 -20||pkd46(lambda red recominase)||||Kirill A. Datsenko PNAS 2000||||||||||require liuzhen's permit
,,antibody,,,,,,,
|-
,109 -20,anti-DsRed polyclonal Antibody,632496-10ul,Clontech ,rabbit antisera,,,,require liuzhen's permit
| ||109 -20||pcp20(eliminate the resistant gene)||||Kirill A. Datsenko PNAS 2000||||||||||require liuzhen's permit
,109 -20,anti-6*His,ab9108,Abcam,,,,,require liuzhen's permit
|-
,109 -20,anti-HA tag,ab9110,Abcam,,,,,require liuzhen's permit
| ||109 -20||psnap||||NEB||||5802bp||||||require liuzhen's permit
,109 -20,Mab to beta prime subunit of E.coli RNA polymerase ,wp001,neoclone ,Epitope 1295-1417(SLAELLNAGLGGS),,,,require liuzhen's permit
|-
,109 -20,Mab to beta subunit of E.coli RNA polymerase ,wp002,neoclone ,Epitope 922-1090,,,,require liuzhen's permit
| ||109 -20||pclipf||||NEB||||5862bp||||||require liuzhen's permit
,109 -20,anti-snap tag antibody,p9310s,NEB,,,,,require liuzhen's permit
|-
,,Dye,,,,,,,
| ||109 -20||IrisFP||||provided by Haodong Chen||||||||||require liuzhen's permit
,109 -20,Yoyo-1,,provided by jianshi jin ,,,,,
|-
,109-20,cy5-dCTP 25nmol,PA55021,GE Healthcare ,,,,,
| ||109 -20||mEos2||||provided by Sunney's group  ||||||||||require liuzhen's permit
,109-20,SNAP-Surface 647,s9136s,NEB,,,,,require liuzhen's permit
|-
,109-20,SNAP-Surface 488,s9124s,NEB,,,,,require liuzhen's permit
| ||109 -20||mEos3.1||||provided by Pingyong Xu||||||||||require liuzhen's permit
,109-20,Clip-surface 488,s9232s,NEB,,,,,require liuzhen's permit
|-
,,regents,,,,,,,
| ||109 -20||mEos3.2||||provided by Pingyong Xu||||||||||require liuzhen's permit
,108 4,mreB perturbing compound A22,sc-204100 lot#12811,Santa Cruz Biotechnology ,,,,,liuzhen
|-
,108 RT,RNA reverse transcription kit ,,Qiagen ,,,,,liuzhen
| ||109 -20||Pdg1-s1(dronpa)||AM-V0071||Amalgaam||||||||||require liuzhen's permit
,109 RT,���۵���֬��,Code D614 lot CB201A,Takara,,,,,liuzhen
|-
,108 4,qPCRmix,,Takara,,,,,liuzhen
| ||109 -20||VAMPZ-ps-cfp2(K+)||||provided by Tianyi yuan from Liangyi Chen's Lab||||||||||require liuzhen's permit
,108 4,dyna beads,,invitrogen ,,,,,liuzhen
|-
,108 4,tetraspec 100nm beads,t7279,invitrogen ,,,,,liuzhen
| ||109 -20||syt7-pa-gfp(A+)||||provided by Tianyi yuan from Liangyi Chen's Lab||||||||||require liuzhen's permit
,108 4,phenymethanesulfonyl fluoride,78830-5G,sigma,,,,,liuzhen
|-
,108 4,lysozyme from chicken egg white ,62970-5g-f,fluka,,,,,liuzhen
| ||109 -20||LOV2-Ja optogenetics ||||from Denise Montell JHCL ||||||||||require liuzhen's permit
,108 4,MEM amino acids solution 50X,11130,invitrogen GIBCO,,,,,liuzhen
|-
,109 -20,DL-Serine hydroxamate,s4503-250mg,sigma ,C3H8N2O3,12011g/mol,,,require liuzhen's permit
| ||109 -20||pUC19 Vector 1mg/ml||N3041S||NEB||||||||||xingdong
,109-20,MEM Vitamin Solution  100X,11120,invitrogen GIBCO,,,,,liuzhen
|-
,-80,E.coli RNA polymerase Holoenzyme ,s90050/s90250,epicentro illumina ,,,,,require liuzhen's permit
| ||109 -20||petduet||71146-3||Novagen||||||||||kongxinyu
,109 4,SNAP-Capture Magnetic Beads,s9145s,NEB,,,,,liuzhen
|-
,109 4,SNAP-Capture Pull Down Resin ,s9144s,NEB,,,,,liuzhen
| ||||strain||||||||||||||
,109 RT,Gene Pulser Cuvette 0.1cm,165-2089,Bio-rad,,,,,liuzhen
|-
,109 RT,Gene Pulser Cuvette 0.2cm,165-2086,Bio-rad,,,,,liuzhen
| ||-80||bw25993||||provided by sunney'group||||||||||require liuzhen's permit
,109 RT,Lithium chloride ,L9650-500G,SIGMA,,,,,liuzhen
|-
,109 RT,QIAquick Gel EXTRACTION Kit(250),28706,Qiagen ,,,,,liuzhen
| ||-80||bw25993+pkd46||||provided by sunney'group||||||||||require liuzhen's permit
,109 RT,Qiagen plasmid Maxi KIT(25),12163,Qiagen ,,,,,liuzhen
|-
,109 RT,Gel DNA recovery Kit,D4008,Zymo,,,,,liuzhen
| ||-80||bl21(ai) ||||provided by sunney'group||||||||||require liuzhen's permit
,109 RT,DNA Clean&Concentator-5,D4013,Zymo,,,,,liuzhen
|-
,109 RT,Qiaprep Spin Miniprep Kit(250),27106,Qiagen ,,,,,liuzhen
| ||-80||BL21(DE3) competent cell||||Transgene||||||||||
,109 RT,QIAquick PCR Purification Kit(50),28104,Qiagen ,,,,,liuzhen
|-
,109 RT,QIAGEN Plasmid Mini kit(25),12123,Qiagen ,,,,,liuzhen
| ||-80||trans5a competent cell||||Transgene||||||||||
,109 RT,QIAGEN Plasmid Mini kit(100),12125,Qiagen ,,,,,liuzhen
|-
,109 RT,calcium chloride ,c5670-100g,sigma,,,2011.11,CaCl2,liuzhen
| ||-80||E.coli strain sx322 resistant to kanamycin ||||provided by sunney'group||||||||||require liuzhen's permit
,109 RT,ammonium chloride,09718-250g,fluka,,,2011.11,NH4CL,liuzhen
|-
,108 4c,polylysine ,,,,,,,
| ||-80||E.coli malI-tetR::eYFP resistant to kanamycin ||||provided by sunney'group||||||||||require liuzhen's permit
,,Enzyme,,,,,,,
|-
,109 -20,phusion high fidelity PCR MMw/HF Buffer ,M0531S,NEB,,,,,liuzhen
| ||-80||6tetO-cam@O2 of Lac,resistant to chloramphenicol ||||provided by sunney'group||||||||||require liuzhen's permit
,109 -20,T4 DNA ligase ,M0202S,NEB,,,,,liuzhen
|-
,109 -20,10X4 DNA ligase buffer ,B0202s,NEB,,,,,liuzhen
| ||||antibody||||||||||||||
,109 -20,KOD plus,12060k5,Toyobo,,,,,liuzhen
|-
,109 -20,KOD Fx,05060c4,Toyobo ,,,,,liuzhen
| ||109 -20||anti-DsRed polyclonal Antibody||632496-10ul||Clontech ||rabbit antisera||||||||require liuzhen's permit
,109 -20,PstI-HF,R3140V,NEB,,,,,xingdong
|-
,109 -20,EcoRI,R0101S,NEB,,,,,xingdong
| ||109 -20||anti-6*His||ab9108||Abcam||||||||||require liuzhen's permit
,109 -20,SphI,R0182S,NEB,,,,,xingdong
|-
,109 -20,DpnI,R0176l,NEB,,,,,xingdong
| ||109 -20||anti-HA tag||ab9110||Abcam||||||||||require liuzhen's permit
,109 -20,HindIII-HF,R3104s,NEB,,,,,xingdong
|-
,109 -20,HindIII,R0104S,NEB,,,,,xingdong
| ||109 -20||Mab to beta prime subunit of E.coli RNA polymerase ||wp001||neoclone ||Epitope 1295-1417(SLAELLNAGLGGS)||||||||require liuzhen's permit
,109 -20,NdeI,R0111S,NEB,,,,,xingdong
|-
,109 -20,BssHI,R0199S,NEB,,,,,xingdong
| ||109 -20||Mab to beta subunit of E.coli RNA polymerase ||wp002||neoclone ||Epitope 922-1090||||||||require liuzhen's permit
,109 -20,BamHI-HF,R3136s,NEB,,,,,xingdong
|-
,109 -20,EcoRI-HF,R3101L,NEB,,,,,xingdong
| ||109 -20||anti-snap tag antibody||p9310s||NEB||||||||||require liuzhen's permit
,109 -20,XhoI,R0146S,NEB,,,,,xingdong
|-
,109 -20,Nb.BbvCI,R0631S,NEB,,,,,xingdong
| ||||Dye||||||||||||||
,109 -20,Bsal-HF,R3535S,NEB,,,,,xingdong
|-
,109 -20,BglII,R0144L,NEB,,,,,xingdong
| ||109 -20||Yoyo-1||||provided by jianshi jin ||||||||||
,109 -20,XbaI,R0145L,NEB,,,,,xingdong
|-
,109 -20,RNaseA,221238BE,Biomed,n,,,,xingdong
| ||109-20||cy5-dCTP 25nmol||PA55021||GE Healthcare ||||||||||
,109 -20,KpnI-HF,R1342S,NEB,,,,,xingdong
|-
,109 -20,NheI-HF,R3131S,NEB,,,,,xingdong
| ||109-20||SNAP-Surface 647||s9136s||NEB||||||||||require liuzhen's permit
,109 -20,NotI,R0189S,NEB,,,,,xingdong
|-
,109 -20,SphI-HF,R3182S,NEB,,,,,xingdong
| ||109-20||SNAP-Surface 488||s9124s||NEB||||||||||require liuzhen's permit
,109 -20,BclI,R0160v,NEB,,,,,xingdong
|-
,109 -20,NEBuffer1,B7001S,NEB,,,,,xingdong
| ||109-20||Clip-surface 488||s9232s||NEB||||||||||require liuzhen's permit
,109 -20,NEBuffer2,B7002S,NEB,,,,,xingdong
|-
,109 -20,NEBuffer3,B7003S,NEB,,,,,xingdong
| ||||regents||||||||||||||
,109 -20,NEBuffer4,B7004S,NEB,,,,,xingdong
|-
,109 -20,Purified BSA 100X,B9001v,NEB,,,,,xingdong
| ||108 4||mreB perturbing compound A22||sc-204100 lot#12811||Santa Cruz Biotechnology ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 RT||RNA reverse transcription kit ||||Qiagen ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||低熔点琼脂糖||Code D614 lot CB201A||Takara||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 4||qPCRmix||||Takara||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 4||dyna beads||||invitrogen ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 4||tetraspec 100nm beads||t7279||invitrogen ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 4||phenymethanesulfonyl fluoride||78830-5G||sigma||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 4||lysozyme from chicken egg white ||62970-5g-f||fluka||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||108 4||MEM amino acids solution 50X||11130||invitrogen GIBCO||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 -20||DL-Serine hydroxamate||s4503-250mg||sigma ||C3H8N2O3||12011g/mol||||||require liuzhen's permit
,,,,,,,,,
|-
,,,,,,,,,
| ||109-20||MEM Vitamin Solution  100X||11120||invitrogen GIBCO||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||-80||E.coli RNA polymerase Holoenzyme ||s90050/s90250||epicentro illumina ||||||||||require liuzhen's permit
,,,,,,,,,
|-
,,,,,,,,,
| ||109 4||SNAP-Capture Magnetic Beads||s9145s||NEB||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 4||SNAP-Capture Pull Down Resin ||s9144s||NEB||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||Gene Pulser Cuvette 0.1cm||165-2089||Bio-rad||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||Gene Pulser Cuvette 0.2cm||165-2086||Bio-rad||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||Lithium chloride ||L9650-500G||SIGMA||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||QIAquick Gel EXTRACTION Kit(250)||28706||Qiagen ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||Qiagen plasmid Maxi KIT(25)||12163||Qiagen ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||Gel DNA recovery Kit||D4008||Zymo||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||DNA Clean&Concentator-5||D4013||Zymo||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||Qiaprep Spin Miniprep Kit(250)||27106||Qiagen ||||||||||liuzhen
,,,,,,,,,
|-
,,,,,,,,,
| ||109 RT||QIAquick PCR Purification Kit(50)||28104||Qiagen ||||||||||liuzhen
|-
| ||109 RT||QIAGEN Plasmid Mini kit(25)||12123||Qiagen ||||||||||liuzhen
|-
| ||109 RT||QIAGEN Plasmid Mini kit(100)||12125||Qiagen ||||||||||liuzhen
|-
| ||109 RT||calcium chloride ||c5670-100g||sigma||||||2011.11||CaCl2||liuzhen
|-
| ||109 RT||ammonium chloride||09718-250g||fluka||||||2011.11||NH4CL||liuzhen
|-
| ||108 4c||polylysine ||||||||||||||
|-
| ||||Enzyme||||||||||||||
|-
| ||109 -20||phusion high fidelity PCR MMw/HF Buffer ||M0531S||NEB||||||||||liuzhen
|-
| ||109 -20||T4 DNA ligase ||M0202S||NEB||||||||||liuzhen
|-
| ||109 -20||10X4 DNA ligase buffer ||B0202s||NEB||||||||||liuzhen
|-
| ||109 -20||KOD plus||12060k5||Toyobo||||||||||liuzhen
|-
| ||109 -20||KOD Fx||05060c4||Toyobo ||||||||||liuzhen
|-
| ||109 -20||PstI-HF||R3140V||NEB||||||||||xingdong
|-
| ||109 -20||EcoRI||R0101S||NEB||||||||||xingdong
|-
| ||109 -20||SphI||R0182S||NEB||||||||||xingdong
|-
| ||109 -20||DpnI||R0176l||NEB||||||||||xingdong
|-
| ||109 -20||HindIII-HF||R3104s||NEB||||||||||xingdong
|-
| ||109 -20||HindIII||R0104S||NEB||||||||||xingdong
|-
| ||109 -20||NdeI||R0111S||NEB||||||||||xingdong
|-
| ||109 -20||BssHI||R0199S||NEB||||||||||xingdong
|-
| ||109 -20||BamHI-HF||R3136s||NEB||||||||||xingdong
|-
| ||109 -20||EcoRI-HF||R3101L||NEB||||||||||xingdong
|-
| ||109 -20||XhoI||R0146S||NEB||||||||||xingdong
|-
| ||109 -20||Nb.BbvCI||R0631S||NEB||||||||||xingdong
|-
| ||109 -20||Bsal-HF||R3535S||NEB||||||||||xingdong
|-
| ||109 -20||BglII||R0144L||NEB||||||||||xingdong
|-
| ||109 -20||XbaI||R0145L||NEB||||||||||xingdong
|-
| ||109 -20||RNaseA||221238BE||Biomed||n||||||||xingdong
|-
| ||109 -20||KpnI-HF||R1342S||NEB||||||||||xingdong
|-
| ||109 -20||NheI-HF||R3131S||NEB||||||||||xingdong
|-
| ||109 -20||NotI||R0189S||NEB||||||||||xingdong
|-
| ||109 -20||SphI-HF||R3182S||NEB||||||||||xingdong
|-
| ||109 -20||BclI||R0160v||NEB||||||||||xingdong
|-
| ||109 -20||NEBuffer1||B7001S||NEB||||||||||xingdong
|-
| ||109 -20||NEBuffer2||B7002S||NEB||||||||||xingdong
|-
| ||109 -20||NEBuffer3||B7003S||NEB||||||||||xingdong
|-
| ||109 -20||NEBuffer4||B7004S||NEB||||||||||xingdong
|-
| ||109 -20||Purified BSA 100X||B9001v||NEB||||||||||xingdong
|-
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|-
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Revision as of 01:54, 22 December 2012

border="1" cellpadding="4" cellspacing="0" style="border:\#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse
序号 存放位置 试剂名称 货号 公司 纯度 分子量 日期 分子式 备注
plasmid Cat.No.
109 -20 pet28a 69864-3 Novagen 5369bp require liuzhen's permit
109 -20 pet21b 69741-3 Novagen 5443bp require liuzhen's permit
109 -20 pbadmychisA V440-01 Invitrogen(kindly provided by Jiwei Liu) 4.1kb require liuzhen's permit
109 -20 pacycduet-1 71147-3 Novagen(kindly provided by Jiwei Liu) 4008bp require liuzhen's permit
-80 pcdfduet-1 71340-3 Novagen 3781bp require liuzhen's permit
109 -20 pkd3(chloramphenicol gene template) Kirill A. Datsenko PNAS 2000 require liuzhen's permit
109 -20 pkd4(kanamycin gene template ) Kirill A. Datsenko PNAS 2000 require liuzhen's permit
109 -20 pkd13(kanamycin gene template ) Kirill A. Datsenko PNAS 2000 require liuzhen's permit
109 -20 pkd46(lambda red recominase) Kirill A. Datsenko PNAS 2000 require liuzhen's permit
109 -20 pcp20(eliminate the resistant gene) Kirill A. Datsenko PNAS 2000 require liuzhen's permit
109 -20 psnap NEB 5802bp require liuzhen's permit
109 -20 pclipf NEB 5862bp require liuzhen's permit
109 -20 IrisFP provided by Haodong Chen require liuzhen's permit
109 -20 mEos2 provided by Sunney's group require liuzhen's permit
109 -20 mEos3.1 provided by Pingyong Xu require liuzhen's permit
109 -20 mEos3.2 provided by Pingyong Xu require liuzhen's permit
109 -20 Pdg1-s1(dronpa) AM-V0071 Amalgaam require liuzhen's permit
109 -20 VAMPZ-ps-cfp2(K+) provided by Tianyi yuan from Liangyi Chen's Lab require liuzhen's permit
109 -20 syt7-pa-gfp(A+) provided by Tianyi yuan from Liangyi Chen's Lab require liuzhen's permit
109 -20 LOV2-Ja optogenetics from Denise Montell JHCL require liuzhen's permit
109 -20 pUC19 Vector 1mg/ml N3041S NEB xingdong
109 -20 petduet 71146-3 Novagen kongxinyu
strain
-80 bw25993 provided by sunney'group require liuzhen's permit
-80 bw25993+pkd46 provided by sunney'group require liuzhen's permit
-80 bl21(ai) provided by sunney'group require liuzhen's permit
-80 BL21(DE3) competent cell Transgene
-80 trans5a competent cell Transgene
-80 E.coli strain sx322 resistant to kanamycin provided by sunney'group require liuzhen's permit
-80 E.coli malI-tetR::eYFP resistant to kanamycin provided by sunney'group require liuzhen's permit
-80 6tetO-cam@O2 of Lac,resistant to chloramphenicol provided by sunney'group require liuzhen's permit
antibody
109 -20 anti-DsRed polyclonal Antibody 632496-10ul Clontech rabbit antisera require liuzhen's permit
109 -20 anti-6*His ab9108 Abcam require liuzhen's permit
109 -20 anti-HA tag ab9110 Abcam require liuzhen's permit
109 -20 Mab to beta prime subunit of E.coli RNA polymerase wp001 neoclone Epitope 1295-1417(SLAELLNAGLGGS) require liuzhen's permit
109 -20 Mab to beta subunit of E.coli RNA polymerase wp002 neoclone Epitope 922-1090 require liuzhen's permit
109 -20 anti-snap tag antibody p9310s NEB require liuzhen's permit
Dye
109 -20 Yoyo-1 provided by jianshi jin
109-20 cy5-dCTP 25nmol PA55021 GE Healthcare
109-20 SNAP-Surface 647 s9136s NEB require liuzhen's permit
109-20 SNAP-Surface 488 s9124s NEB require liuzhen's permit
109-20 Clip-surface 488 s9232s NEB require liuzhen's permit
regents
108 4 mreB perturbing compound A22 sc-204100 lot#12811 Santa Cruz Biotechnology liuzhen
108 RT RNA reverse transcription kit Qiagen liuzhen
109 RT 低熔点琼脂糖 Code D614 lot CB201A Takara liuzhen
108 4 qPCRmix Takara liuzhen
108 4 dyna beads invitrogen liuzhen
108 4 tetraspec 100nm beads t7279 invitrogen liuzhen
108 4 phenymethanesulfonyl fluoride 78830-5G sigma liuzhen
108 4 lysozyme from chicken egg white 62970-5g-f fluka liuzhen
108 4 MEM amino acids solution 50X 11130 invitrogen GIBCO liuzhen
109 -20 DL-Serine hydroxamate s4503-250mg sigma C3H8N2O3 12011g/mol require liuzhen's permit
109-20 MEM Vitamin Solution 100X 11120 invitrogen GIBCO liuzhen
-80 E.coli RNA polymerase Holoenzyme s90050/s90250 epicentro illumina require liuzhen's permit
109 4 SNAP-Capture Magnetic Beads s9145s NEB liuzhen
109 4 SNAP-Capture Pull Down Resin s9144s NEB liuzhen
109 RT Gene Pulser Cuvette 0.1cm 165-2089 Bio-rad liuzhen
109 RT Gene Pulser Cuvette 0.2cm 165-2086 Bio-rad liuzhen
109 RT Lithium chloride L9650-500G SIGMA liuzhen
109 RT QIAquick Gel EXTRACTION Kit(250) 28706 Qiagen liuzhen
109 RT Qiagen plasmid Maxi KIT(25) 12163 Qiagen liuzhen
109 RT Gel DNA recovery Kit D4008 Zymo liuzhen
109 RT DNA Clean&Concentator-5 D4013 Zymo liuzhen
109 RT Qiaprep Spin Miniprep Kit(250) 27106 Qiagen liuzhen
109 RT QIAquick PCR Purification Kit(50) 28104 Qiagen liuzhen
109 RT QIAGEN Plasmid Mini kit(25) 12123 Qiagen liuzhen
109 RT QIAGEN Plasmid Mini kit(100) 12125 Qiagen liuzhen
109 RT calcium chloride c5670-100g sigma 2011.11 CaCl2 liuzhen
109 RT ammonium chloride 09718-250g fluka 2011.11 NH4CL liuzhen
108 4c polylysine
Enzyme
109 -20 phusion high fidelity PCR MMw/HF Buffer M0531S NEB liuzhen
109 -20 T4 DNA ligase M0202S NEB liuzhen
109 -20 10X4 DNA ligase buffer B0202s NEB liuzhen
109 -20 KOD plus 12060k5 Toyobo liuzhen
109 -20 KOD Fx 05060c4 Toyobo liuzhen
109 -20 PstI-HF R3140V NEB xingdong
109 -20 EcoRI R0101S NEB xingdong
109 -20 SphI R0182S NEB xingdong
109 -20 DpnI R0176l NEB xingdong
109 -20 HindIII-HF R3104s NEB xingdong
109 -20 HindIII R0104S NEB xingdong
109 -20 NdeI R0111S NEB xingdong
109 -20 BssHI R0199S NEB xingdong
109 -20 BamHI-HF R3136s NEB xingdong
109 -20 EcoRI-HF R3101L NEB xingdong
109 -20 XhoI R0146S NEB xingdong
109 -20 Nb.BbvCI R0631S NEB xingdong
109 -20 Bsal-HF R3535S NEB xingdong
109 -20 BglII R0144L NEB xingdong
109 -20 XbaI R0145L NEB xingdong
109 -20 RNaseA 221238BE Biomed n xingdong
109 -20 KpnI-HF R1342S NEB xingdong
109 -20 NheI-HF R3131S NEB xingdong
109 -20 NotI R0189S NEB xingdong
109 -20 SphI-HF R3182S NEB xingdong
109 -20 BclI R0160v NEB xingdong
109 -20 NEBuffer1 B7001S NEB xingdong
109 -20 NEBuffer2 B7002S NEB xingdong
109 -20 NEBuffer3 B7003S NEB xingdong
109 -20 NEBuffer4 B7004S NEB xingdong
109 -20 Purified BSA 100X B9001v NEB xingdong