BE.109:Bio-material engineering: Difference between revisions
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'''Instructors:''' Angela Belcher and [[Natalie Kuldell]] | '''Instructors:''' Angela Belcher and [[Natalie Kuldell]] | ||
'''TA:''' Eileen Higham | '''TA:''' [[Eileen Higham]] | ||
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | ||
Revision as of 09:00, 11 January 2006
Module 4
Instructors: Angela Belcher and Natalie Kuldell
TA: Eileen Higham
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Lab handouts
Day 2: Panning for gold binders
Day 3: Screening library and optimizing panning
Day 5: PCR of gold binding candidates
References
Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.
- Design criteria for engineering inorganic material-specific peptides.
Langmuir 2005 21(15):6929-6933
Peelle BR, Krauland EM, Wittrup KD, Belcher AM
URL PDF reprint first supplement to pdf second supplement to pdf
