BCH081006-Construction of pJ23006-J23061 key/tRNA

From OpenWetWare
Revision as of 17:19, 10 August 2006 by JCAnderson (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

Overlap extension KB032/KB033 (141bp, XbaI/PstI)
Sub into pJ23006 (XbaI/PstI)
Product is pJ23006-J23061, screen with XhoI/AlwNI

KB032
cctagtctagagggagagatgccggagcggctgaacggaccgggatccacCTCGAGctaagcttcccg
KB033
ctgaactgcagcggccgctactagtatggcggagagagggggatttgaacccccggtagagttgcccctactccgggaagcttagCTCGAGg

Retrieved from "https://openwetware.org/mediawiki/index.php?title=BCH081006-Construction_of_pJ23006-J23061_key/tRNA&oldid=59259"