B-Per II: Difference between revisions
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B-Per and B-Per II are reagents | ==Purpose== | ||
B-Per and B-Per II are reagents for bacterial lysis. It is a gentle reagent that can be used to lyse cells without denaturing proteins. It has been used to lyse ''E. coli'' cells to measure the GFP concentration via the [[Endy:Victor3 plate reader|Endy lab plate reader]]. | |||
==Procurement== | |||
B-Per and B-Per II are sold by Pierce ([http://www.piercenet.com). | |||
==Use== | |||
For a usage protocol developed by [[Ania Labno]] and [[Barry Canton]], see this [[Endy:Victor3 plate reader/Calibrating the GFP-separated label|page]]. | |||
==Safety== | |||
B-Per is an irritant. | |||
==References== | |||
*[http://www.piercenet.com/poppers/nonanimated/bper/index.html Product webpage] | *[http://www.piercenet.com/poppers/nonanimated/bper/index.html Product webpage] | ||
*[http://www.piercenet.com/resources/browse.cfm?fldID=1D083447-2515-49E1-BFD7-D2A7341B899A Product FAQ] | *[http://www.piercenet.com/resources/browse.cfm?fldID=1D083447-2515-49E1-BFD7-D2A7341B899A Product FAQ] | ||
Revision as of 10:25, 4 June 2006
Purpose
B-Per and B-Per II are reagents for bacterial lysis. It is a gentle reagent that can be used to lyse cells without denaturing proteins. It has been used to lyse E. coli cells to measure the GFP concentration via the Endy lab plate reader.
Procurement
B-Per and B-Per II are sold by Pierce ([http://www.piercenet.com).
Use
For a usage protocol developed by Ania Labno and Barry Canton, see this page.
Safety
B-Per is an irritant.
