This page has been "stolen" from http://openwetware.org/wiki/Arking:JCAOligoTutorialHome. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. And until I am done, I hope J.C. Anderson will keep his valuable pages online.
Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.
Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.