Axel:Schwekendiek Tutorials: Difference between revisions
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The material on this page has been "stolen" from [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Prof. John C. Anderson at UC Berkeley]. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon [[Image:RecentChanges_iconbaby.png]]. Until this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information. | The material on this page has been "stolen" from [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Prof. John C. Anderson at UC Berkeley]. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon [[Image:RecentChanges_iconbaby.png]]. Until this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information. Pages marked with [[Image:GettingStarted_iconbaby.png]] are a "must read"! | ||
==The Basics== | ==The Basics== | ||
'''Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.''' | '''Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics. ''The material in this section needs to be understood by everyone before starting lab work. It is essential. But I assume this knowledge is present from other courses and I won't go over it (except upon request).''''' | ||
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==Introduction to Biobricks and Basic Cloning== | ==Introduction to Biobricks and Basic Cloning== | ||
'''This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it | '''This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it. ''' | ||
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[[Arking:JCATutorialIntro9 | Installing ApE]] | [[Arking:JCATutorialIntro9 | Installing ApE]] - this program is not required for the UNI course! | ||
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[[Arking:JCATutorialIntro7 | Overview of Cloning]] | [[Arking:JCATutorialIntro7 | Overview of Cloning]] [[Image:GettingStarted_iconbaby.png]] | ||
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[[Arking:JCATutorialIntro1 | Introduction to Biobricks]] | [[Arking:JCATutorialIntro1 | Introduction to Biobricks]] [[Image:GettingStarted_iconbaby.png]] | ||
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[[Axel:Schwekendiek_Working_with_bacterial_stocks | Working with bacterial stocks]] [[Image:RecentChanges_iconbaby.png]][[Image:GettingStarted_iconbaby.png]] | |||
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==Technical Design== | ==Technical Design== | ||
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[[Arking:JCAOligoTutorial1 | Basic Oligo Design]] | [[Arking:JCAOligoTutorial1 | Basic Oligo Design]] [[Image:GettingStarted_iconbaby.png]] | ||
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==Implementation== | ==Implementation== | ||
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[[Axel:Schwekendiek_Tutorial1 | Basic Gene Recombination]] | [[Axel:Schwekendiek_Tutorial1 | Basic Gene Recombination]] [[Image:GettingStarted_iconbaby.png]] | ||
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==Introduction to Pathway Engineering== | ==Introduction to Pathway Engineering== | ||
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coming | coming sooner | ||
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coming | coming soon | ||
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==Things you should know== | ==Things you should know== | ||
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[[Arking:JCAOligoTutorial7 | Common Pitfalls and Tips]] | [[Arking:JCAOligoTutorial7 | Common Pitfalls and Tips]] [[Image:GettingStarted_iconbaby.png]] | ||
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Latest revision as of 14:41, 20 August 2009
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Schwekendiek Lab - University of Northern Iowa |
|---|
The material on this page has been "stolen" from Prof. John C. Anderson at UC Berkeley. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon 
. Until this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information. Pages marked with 
are a "must read"!
The Basics
Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics. The material in this section needs to be understood by everyone before starting lab work. It is essential. But I assume this knowledge is present from other courses and I won't go over it (except upon request).
Bare bones biochemistry
Basic Molecular Biology
Practical DNA
What is PCR?
Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.
Installing ApE - this program is not required for the UNI course!
Overview of Cloning
Introduction to Biobricks
Working with bacterial stocks
Introduction to Refactoring
Technical Design
Basic Oligo Design
Special Case: Single Internal Restriction Sites
Construction of short parts
Design of composite parts
Keeping track of construction files
Sequencing Analysis
Advanced Technical Design
Working with Translational Fusions
Circularly Permuted Proteins
The "what did they make" game
Implementation
Introduction to Pathway Engineering
coming sooner
coming soon
Things you should know
Common Pitfalls and Tips
Origins of replication
The Critical Path
