Axel:Schwekendiek Tutorials

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The material on this page has been "stolen" from [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Prof. John C. Anderson at UC Berkeley]. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon [[Image:Service_lab.png]]. And this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information.
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The material on this page has been "stolen" from [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Prof. John C. Anderson at UC Berkeley]. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon [[Image:RecentChanges_iconbaby.png]]. Until this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information. Pages marked with [[Image:GettingStarted_iconbaby.png]] are a "must read"!
==The Basics==
==The Basics==
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'''Think the "Central Dogma" involves a kennel of chihuahuas?  You need to start here with the basics.'''
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'''Think the "Central Dogma" involves a kennel of chihuahuas?  You need to start here with the basics. ''The material in this section needs to be understood by everyone before starting lab work. It is essential. But I assume this knowledge is present from other courses and I won't go over it (except upon request).'''''
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==Introduction to Biobricks and Basic Cloning==
==Introduction to Biobricks and Basic Cloning==
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'''This tutorial is best used in conjunction with the sequence editor ApE.  Read the section about ApE for a description of how to install it.'''
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'''This tutorial is best used in conjunction with the sequence editor ApE.  Read the section about ApE for a description of how to install it. '''
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[[Arking:JCATutorialIntro9 | Installing ApE]]
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[[Arking:JCATutorialIntro9 | Installing ApE]] - this program is not required for the UNI course!
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[[Arking:JCATutorialIntro7 | Overview of Cloning]]
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[[Arking:JCATutorialIntro7 | Overview of Cloning]] [[Image:GettingStarted_iconbaby.png]]
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[[Arking:JCATutorialIntro1 | Introduction to Biobricks]]
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[[Arking:JCATutorialIntro1 | Introduction to Biobricks]] [[Image:GettingStarted_iconbaby.png]]
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[[Axel:Schwekendiek_Working_with_bacterial_stocks | Working with bacterial stocks]] [[Image:RecentChanges_iconbaby.png]][[Image:GettingStarted_iconbaby.png]]
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==Technical Design==
==Technical Design==
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[[Arking:JCAOligoTutorial1 | Basic Oligo Design]]
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[[Arking:JCAOligoTutorial1 | Basic Oligo Design]] [[Image:GettingStarted_iconbaby.png]]
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==Implementation==
==Implementation==
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[[Axel:Schwekendiek_Tutorial1 | Basic Gene Recombination]]
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[[Axel:Schwekendiek_Tutorial1 | Basic Gene Recombination]] [[Image:GettingStarted_iconbaby.png]]
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==Introduction to Pathway Engineering==
==Introduction to Pathway Engineering==
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==Things you should know==
==Things you should know==
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[[Arking:JCAOligoTutorial7 | Common Pitfalls and Tips]]
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[[Arking:JCAOligoTutorial7 | Common Pitfalls and Tips]] [[Image:GettingStarted_iconbaby.png]]
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Current revision

Schwekendiek Lab - University of Northern Iowa



The material on this page has been "stolen" from Prof. John C. Anderson at UC Berkeley. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. Updated tutorials will be marked with this icon Image:RecentChanges_iconbaby.png. Until this happens, I hope John will keep his valuable pages online and the links working. If the links become dead, please let me know and will hurry replacing the information. Pages marked with Image:GettingStarted_iconbaby.png are a "must read"!

The Basics

Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics. The material in this section needs to be understood by everyone before starting lab work. It is essential. But I assume this knowledge is present from other courses and I won't go over it (except upon request).

Bare bones biochemistry

Basic Molecular Biology

Practical DNA

What is PCR?

Introduction to Biobricks and Basic Cloning

This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.

Installing ApE - this program is not required for the UNI course!

Overview of Cloning Image:GettingStarted_iconbaby.png

Introduction to Biobricks Image:GettingStarted_iconbaby.png

Working with bacterial stocks Image:RecentChanges_iconbaby.pngImage:GettingStarted_iconbaby.png

Introduction to Refactoring

Technical Design

Basic Oligo Design Image:GettingStarted_iconbaby.png

Special Case: Single Internal Restriction Sites

Construction of short parts

Design of composite parts

Keeping track of construction files

Sequencing Analysis

Advanced Technical Design

Working with Translational Fusions

Circularly Permuted Proteins

The "what did they make" game

Implementation

Basic Gene Recombination Image:GettingStarted_iconbaby.png

Introduction to Pathway Engineering

coming sooner

coming soon

Things you should know

Common Pitfalls and Tips Image:GettingStarted_iconbaby.png

Origins of replication

The Critical Path

Personal tools