Aseptic Technique - Revision history

Alyssa Chase: /* Aseptic Technique */

Alyssa Chase — Tue, 08 Sep 2009 20:05:40 GMT

Aseptic Technique

←Older revision		Revision as of 20:05, 8 September 2009
Line 43:		Line 43:
	* Never perform mouth pipetting. Pipettor must be used.		* Never perform mouth pipetting. Pipettor must be used.

-	* When handling sterile containers with caps or lids, place the cap on its side if it must be laid	+	* When handling sterile containers with caps or lids, place the cap on its side if it must be laid on the work surface.
-	on the work surface.	+

	* Make sure not to touch the tip of the pipette to the rim of any flask or sterile bottle.		* Make sure not to touch the tip of the pipette to the rim of any flask or sterile bottle.

	* Clean the work area when finished by wiping with 70% alcohol.		* Clean the work area when finished by wiping with 70% alcohol.

Alyssa Chase at 20:04, 8 September 2009

Alyssa Chase — Tue, 08 Sep 2009 20:04:35 GMT

←Older revision		Revision as of 20:04, 8 September 2009
Line 11:		Line 11:
	• Swab down the working surface liberally with 70% ethanol.		• Swab down the working surface liberally with 70% ethanol.

-	• Periodically spread a solution of 70% ethanol over the exterior of gloves to minimize	+	• Periodically spread a solution of 70% ethanol over the exterior of gloves to minimize contamination. Replace them if torn.
-		+
-	contamination. Replace them if torn.	+

	• In case of any spill, spread a solution of 70% alcohol and swab immediately with non-linting		• In case of any spill, spread a solution of 70% alcohol and swab immediately with non-linting
-		+	wipes.
-	wipes.	+

	• Discard gloves after use and do not wear them when entering any other lab area. Bring into the		• Discard gloves after use and do not wear them when entering any other lab area. Bring into the
-		+	work area only those items needed for a particular procedure.
-	work area only those items needed for a particular procedure.	+

	• Leave a wide clear space in the center of the hood (not just the front edge) to work on. Do not		• Leave a wide clear space in the center of the hood (not just the front edge) to work on. Do not
-		+	clutter the area to prevent blockage of proper air flow and to minimize turbulence.
-	clutter the area to prevent blockage of proper air flow and to minimize turbulence.	+

	• Swab with 70% alcohol all glassware(medium bottles, beakers, etc.)before placing them inside the		• Swab with 70% alcohol all glassware(medium bottles, beakers, etc.)before placing them inside the
-		+	hood.
-	hood.	+

	• Arrange the work area to have easy access to all of it without having to reach over one item to		• Arrange the work area to have easy access to all of it without having to reach over one item to
-		+	get at another (especially over an open bottle or flask).
-	get at another (especially over an open bottle or flask).	+

	• Use sterile wrapped pipettes and discard them after use into a biohazard waste container.		• Use sterile wrapped pipettes and discard them after use into a biohazard waste container.
Line 51:		Line 44:

	* When handling sterile containers with caps or lids, place the cap on its side if it must be laid		* When handling sterile containers with caps or lids, place the cap on its side if it must be laid
-		+	on the work surface.
-	on the work surface.	+

	* Make sure not to touch the tip of the pipette to the rim of any flask or sterile bottle.		* Make sure not to touch the tip of the pipette to the rim of any flask or sterile bottle.

	* Clean the work area when finished by wiping with 70% alcohol.		* Clean the work area when finished by wiping with 70% alcohol.

Alyssa Chase at 19:59, 8 September 2009

Alyssa Chase — Tue, 08 Sep 2009 19:59:47 GMT

Alyssa Chase at 19:57, 8 September 2009

Alyssa Chase — Tue, 08 Sep 2009 19:57:37 GMT

←Older revision		Revision as of 19:57, 8 September 2009
Line 1:		Line 1:
-	Aseptic Technique	+	= Aseptic Technique =
		+
		+	• Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material

-	~~• Protect eyes, mucous membranes, open cuts and wounds from contact with~~
-	~~biohazard material.~~
	• Use gloves, goggles, mask, and protective clothing as necessary.		• Use gloves, goggles, mask, and protective clothing as necessary.
		+
	• Carry out all culturing operation in a laminar flow hood.		• Carry out all culturing operation in a laminar flow hood.
		+
	• Disinfect all surfaces prior to use with a disinfectant solution.		• Disinfect all surfaces prior to use with a disinfectant solution.
		+
	• Swab down the working surface liberally with 70% ethanol.		• Swab down the working surface liberally with 70% ethanol.
-	• Periodically spread a solution of 70% ethanol over the exterior of gloves to	+
-	~~minimize~~ contamination. Replace them if torn.	+	• Periodically spread a solution of 70% ethanol over the exterior of gloves to minimize
-	• In case of any spill, spread a solution of 70% alcohol and swab immediately with	+	contamination. Replace them if torn.
-	non-linting wipes.	+
-	• Discard gloves after use and do not wear them when entering any other lab area.	+	• In case of any spill, spread a solution of 70% alcohol and swab immediately with non-linting
-	Bring into the work area only those items needed for a particular procedure.	+	wipes.
-	• Leave a wide clear space in the center of the hood (not just the front edge) to work	+
-	on. Do not clutter the area to prevent blockage of proper air flow and to	+	• Discard gloves after use and do not wear them when entering any other lab area. Bring into the
-	minimize turbulence.	+	work area only those items needed for a particular procedure.
-	• Swab with 70% alcohol all glassware (medium bottles, beakers, etc.) before	+
-	placing them inside the hood.	+	• Leave a wide clear space in the center of the hood (not just the front edge) to work on. Do not
-	• Arrange the work area to have easy access to all of it without having to reach	+	clutter the area to prevent blockage of proper air flow and to minimize turbulence.
-	over one item to get at another (especially over an open bottle or flask).	+
-	• Use sterile wrapped pipettes and discard them after use into a biohazard waste	+	• Swab with 70% alcohol all glassware(medium bottles, beakers, etc.)before placing them inside the
-	container.	+	hood.
		+
		+	• Arrange the work area to have easy access to all of it without having to reach over one item to
		+	get at another (especially over an open bottle or flask).
		+
		+	• Use sterile wrapped pipettes and discard them after use into a biohazard waste container.
		+
	• Check that the wrapping of the sterile pipette is not broken or damaged.		• Check that the wrapping of the sterile pipette is not broken or damaged.
		+
	• Inspect the vessels to be used:		• Inspect the vessels to be used:
-	• T-flask – Must be free from visible contamination or breakage, or lack container	+
-	identification. The plastic covering the flask must be intact.	+	a. T-flask – Must be free from visible contamination or breakage, or lack container
-	• Bottles – Check for cracks, expiration dates.	+	identification. The plastic covering the flask must be intact.
-	• Spinners flasks – Check for cracks, expiration dates, and proper assembly.	+	b. Bottles – Check for cracks, expiration dates.
-	• Discard any biohazard or contaminated material immediately.	+	c. Spinners flasks – Check for cracks, expiration dates, and proper assembly.
-	• Never perform mouth pipetting. Pipettor must be used.	+
-	• When handling sterile containers with caps or lids, place the cap on its side if it	+	* Discard any biohazard or contaminated material immediately.
-	must be laid on the work surface.	+
-	• Make sure not to touch the tip of the pipette to the rim of any flask or sterile	+	* Never perform mouth pipetting. Pipettor must be used.
-	bottle.	+
-	• Clean the work area when finished by wiping with 70% alcohol.	+	* When handling sterile containers with caps or lids, place the cap on its side if it must be laid
		+	on the work surface.
		+
		+	* Make sure not to touch the tip of the pipette to the rim of any flask or sterile bottle.
		+
		+	* Clean the work area when finished by wiping with 70% alcohol.

Kehinde Kassim: New page: Aseptic Technique • Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material. • Use gloves, goggles, mask, and protective clothing as necessary. ...

Kehinde Kassim — Tue, 08 Sep 2009 18:21:33 GMT

New page: Aseptic Technique • Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material. • Use gloves, goggles, mask, and protective clothing as necessary. ...

New page

Aseptic Technique

• Protect eyes, mucous membranes, open cuts and wounds from contact with
biohazard material.
• Use gloves, goggles, mask, and protective clothing as necessary.
• Carry out all culturing operation in a laminar flow hood.
• Disinfect all surfaces prior to use with a disinfectant solution.
• Swab down the working surface liberally with 70% ethanol.
• Periodically spread a solution of 70% ethanol over the exterior of gloves to
minimize contamination. Replace them if torn.
• In case of any spill, spread a solution of 70% alcohol and swab immediately with
non-linting wipes.
• Discard gloves after use and do not wear them when entering any other lab area.
Bring into the work area only those items needed for a particular procedure.
• Leave a wide clear space in the center of the hood (not just the front edge) to work
on. Do not clutter the area to prevent blockage of proper air flow and to
minimize turbulence.
• Swab with 70% alcohol all glassware (medium bottles, beakers, etc.) before
placing them inside the hood.
• Arrange the work area to have easy access to all of it without having to reach
over one item to get at another (especially over an open bottle or flask).
• Use sterile wrapped pipettes and discard them after use into a biohazard waste
container.
• Check that the wrapping of the sterile pipette is not broken or damaged.
• Inspect the vessels to be used:
• T-flask – Must be free from visible contamination or breakage, or lack container
identification. The plastic covering the flask must be intact.
• Bottles – Check for cracks, expiration dates.
• Spinners flasks – Check for cracks, expiration dates, and proper assembly.
• Discard any biohazard or contaminated material immediately.
• Never perform mouth pipetting. Pipettor must be used.
• When handling sterile containers with caps or lids, place the cap on its side if it
must be laid on the work surface.
• Make sure not to touch the tip of the pipette to the rim of any flask or sterile
bottle.
• Clean the work area when finished by wiping with 70% alcohol.

←Older revision		Revision as of 19:59, 8 September 2009
Line 11:		Line 11:
	• Swab down the working surface liberally with 70% ethanol.		• Swab down the working surface liberally with 70% ethanol.

-	• Periodically spread a solution of 70% ethanol over the exterior of gloves to minimize	+	• Periodically spread a solution of 70% ethanol over the exterior of gloves to minimize
		+
	contamination. Replace them if torn.		contamination. Replace them if torn.

	• In case of any spill, spread a solution of 70% alcohol and swab immediately with non-linting		• In case of any spill, spread a solution of 70% alcohol and swab immediately with non-linting
		+
	wipes.		wipes.

	• Discard gloves after use and do not wear them when entering any other lab area. Bring into the		• Discard gloves after use and do not wear them when entering any other lab area. Bring into the
		+
	work area only those items needed for a particular procedure.		work area only those items needed for a particular procedure.

	• Leave a wide clear space in the center of the hood (not just the front edge) to work on. Do not		• Leave a wide clear space in the center of the hood (not just the front edge) to work on. Do not
		+
	clutter the area to prevent blockage of proper air flow and to minimize turbulence.		clutter the area to prevent blockage of proper air flow and to minimize turbulence.

	• Swab with 70% alcohol all glassware(medium bottles, beakers, etc.)before placing them inside the		• Swab with 70% alcohol all glassware(medium bottles, beakers, etc.)before placing them inside the
		+
	hood.		hood.

	• Arrange the work area to have easy access to all of it without having to reach over one item to		• Arrange the work area to have easy access to all of it without having to reach over one item to
		+
	get at another (especially over an open bottle or flask).		get at another (especially over an open bottle or flask).

Line 45:		Line 51:

	* When handling sterile containers with caps or lids, place the cap on its side if it must be laid		* When handling sterile containers with caps or lids, place the cap on its side if it must be laid
		+
	on the work surface.		on the work surface.