Arrest in G1 phase using a/alpha Mating Factor: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
'''Protocol''' | '''Protocol''' | ||
1) Grow yeast | 1) Grow yeast culture to desired density. | ||
2) Add yeast mating factor to a final concentration of 2.5 ug/mL. | 2) Add yeast mating factor to a final concentration of 2.5 ug/mL. | ||
Revision as of 23:19, 25 February 2006
Protocol
1) Grow yeast culture to desired density.
2) Add yeast mating factor to a final concentration of 2.5 ug/mL.
If growing a-type yeast, use alpha mating factor. For alpha-type yeast, use a mating factor.
3) Incubate at 30 C for 3 hours. The yeast should form "shmoos" and look pear-shaped once arrested.
