Arking:JCAOligoTutorialHome
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Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE (A plasmid Editor). It can be downloaded from http://www.biology.utah.edu/jorgensen/wayned/ape/
Experimental Design
Basic Oligo Design
Special Case: Single Internal Restriction Sites
Design of composite parts
Experimental Implementation
Implementing the Basic Construction File
DBBS Composite Part Assembly
Sequencing Analysis
If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com
