Arking:JCAOligoTutorialHome
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Basic Cloning and Biobrick Assembly Tutorial |
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The Basics
Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.
Bare bones biochemistry
Basic Molecular Biology
Practical DNA
What is PCR?
Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.
Installing ApE
Overview of Cloning
Introduction to Biobricks
Introduction to Refactoring
Technical Design
Basic Oligo Design
Special Case: Single Internal Restriction Sites
Construction of short parts
Working with Translational Fusions
Design of composite parts
Keeping track of construction files
Sequencing Analysis
Circular Permuted Proteins
Implementation
Implementing the Basic Construction File
Things you should know
Common Pitfalls and Tips
Origins of replication
The Critical Path
If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com
