Arking:JCAOligoTutorialHome: Difference between revisions
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==Introduction to Biobricks and Basic Cloning== | ==Introduction to Biobricks and Basic Cloning== | ||
'''This tutorial is best used in conjunction with the sequence editor ApE (A plasmid Editor). It can be downloaded from http://www.biology.utah.edu/jorgensen/wayned/ape/ ''' | '''This tutorial is best used in conjunction with the sequence editor ApE (A plasmid Editor). It can be downloaded from http://www.biology.utah.edu/jorgensen/wayned/ape/ '''. Read the section about ApE for a description of how to install it and what its supposed to do. | ||
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[[Arking:JCATutorialIntro9 | Installing and using ApE]] | |||
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<span style='font-size:18.0pt'> | <span style='font-size:18.0pt'> | ||
[[Arking:JCATutorialIntro7 | Overview of Cloning]] | [[Arking:JCATutorialIntro7 | Overview of Cloning]] | ||
Revision as of 16:56, 28 May 2007
Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE (A plasmid Editor). It can be downloaded from http://www.biology.utah.edu/jorgensen/wayned/ape/ . Read the section about ApE for a description of how to install it and what its supposed to do.
Installing and using ApE
Overview of Cloning
Introduction to Biobricks
Introduction to Refactoring
Experimental Design
Basic Oligo Design
Special Case: Single Internal Restriction Sites
Design of composite parts
Sequencing Analysis
Experimental Implementation
Implementing the Basic Construction File
DBBS Composite Part Assembly
If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com
