Arking:JCAOligoTutoria23: Difference between revisions
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===From PMID: === | ===From PMID: === | ||
*Rewrite this as a construction file | |||
*Predict the product | *Predict the product | ||
*How big is it? (I get ###### bp) | *How big is it? (I get ###### bp) | ||
===From PMID: === | ===From PMID: === | ||
*Predict the product | *Predict the product | ||
*How big is it? (I get ###### bp) | *How big is it? (I get ###### bp) | ||
Revision as of 19:13, 10 February 2009
From PMID: 18045411
Construction of expression plasmid The coding region of the C. thermophilum glucoamylase gene was amplified by PCR, using the following oligonucleotide primers: gla-ep5 5¢-GCGTACGTAGCGGTCGAT TCCTACATTG-3¢ (SnaBI) and gla-ep3 5¢-GTCGCGGCC GCTCACCAGTGGTCTTGACCAC-3¢ (NotI). To facilitate the cloning of the amplified fragment, the sense and antisense primers contain a SnaBI and a NotI restriction site respectively. The amplified product was digested with SnaBI and NotI and ligated to pPIC9K (also digested with SnaBI and a NotI), producing the P. pastoris secretion expression plasmid pPIC9K-gla (Fig. 1). The DNA manipulations were carried out using standard procedures (Sambrook et al. 1989).
- Rewrite this as a construction file
- Predict the product
- How big is it? (I get 10974 bp)
From PMID:
- Rewrite this as a construction file
- Predict the product
- How big is it? (I get ###### bp)
From PMID:
- Predict the product
- How big is it? (I get ###### bp)
