Antarctic Phosphatase

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Website

NEB's Antarctic Phosphatase

Associated protocols

Phosphatase treatment of linearized vector

Notes

This enzyme is preferable to either Calf Intestinal Phosphatase or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.

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