Antarctic Phosphatase - Revision history

David Gorczyca: /* Notes */

2011-08-19T02:34:03Z

Notes

←Older revision		Revision as of 02:34, 19 August 2011
Line 9:		Line 9:
	==Notes==		==Notes==

-	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.	+	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.

	[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]

David Gorczyca: /* Notes */

2011-08-19T02:33:37Z

Notes

←Older revision		Revision as of 02:33, 19 August 2011
Line 9:		Line 9:
	==Notes==		==Notes==

-	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. ~~Contaminating enzymatic~~ activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing successful ~~ligation~~. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.	+	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.

	[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]

David Gorczyca: /* Notes */

2011-08-19T02:32:11Z

Notes

←Older revision		Revision as of 02:32, 19 August 2011
Line 9:		Line 9:
	==Notes==		==Notes==

-	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.	+	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. Contaminating enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing successful ligation. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.

	[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]

Reshma P. Shetty at 14:21, 24 May 2007

2007-05-24T14:21:18Z

←Older revision		Revision as of 14:21, 24 May 2007
Line 10:		Line 10:

	This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.		This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.
		+
		+	[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]

Reshma P. Shetty at 16:55, 31 May 2005

2005-05-31T16:55:29Z

New page

==Website==

[http://www.neb.com/nebecomm/products/productM0289.asp NEB's Antarctic Phosphatase]

==Associated protocols==

[[Phosphatase treatment of linearized vector]]

==Notes==

This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.