Antarctic Phosphatase: Difference between revisions
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==Notes== | ==Notes== | ||
This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K. | This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K. | ||
[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]] | [[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]] | ||
Latest revision as of 19:34, 18 August 2011
Website
Associated protocols
Phosphatase treatment of linearized vector
Notes
This enzyme is preferable to either Calf Intestinal Phosphatase or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.
