Antarctic Phosphatase

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(Notes)
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==Notes==
==Notes==
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This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.
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This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.  Contaminating enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing successful ligation.  Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.
[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]
[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]

Revision as of 21:32, 18 August 2011

Website

NEB's Antarctic Phosphatase

Associated protocols

Phosphatase treatment of linearized vector

Notes

This enzyme is preferable to either Calf Intestinal Phosphatase or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. Contaminating enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing successful ligation. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.

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