Annexin-V

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  • Conjugated to other fluorochromes (PE, FITC)
  • Stains cells in early stage of apoptosis
    • Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
  • Must be used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)
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