Annexin-V - Revision history

Jakob Suckale: categories

Jakob Suckale — Tue, 05 May 2009 13:29:24 GMT

Benjamin E. Epstein at 07:55, 8 July 2008

Benjamin E. Epstein — Tue, 08 Jul 2008 07:55:41 GMT

←Older revision		Revision as of 07:55, 8 July 2008
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-	* Conjugated to other fluorochromes (PE, FITC)	+	==Purpose==
	* Stains cells in '''early stage of apoptosis'''		* Stains cells in '''early stage of apoptosis'''
	** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis		** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
-	* ~~Must be used~~ in conjunction with vital dye (e.g. [[Propidium iodide\|PI]], [[7-AAD]]) to distinguish dead cells (Annexin-V +/7-AAD +~~) from apoptotic cells (~~Annexin-V +/7-AAD -)	+
		+	==Procurement==
		+	* Standard companies
		+
		+	==Use==
		+	* Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
		+	* Used in conjunction with vital dye (e.g. [[Propidium iodide\|PI]], [[7-AAD]]) to distinguish dead cells from apoptotic cells
		+	** Annexin-V +/7-AAD +: Dead
		+	** Annexin-V +/7-AAD -: Apoptotic
		+	* [http://www.bdbiosciences.com/pharmingen/protocols/Annexin_V_Staining.shtml BD Biosciences protocol]
		+	<!-- *Include any relevant links to [[Protocols\|OWW protocols]]
		+	*Include any relevant links to the manufacturer's protocols -->
		+
		+	==Safety==
		+	* No significant considerations ([http://www.bdbiosciences.com/external_files/pm/doc/msds/live/web_enabled/556417-MSDS.00.pdf BD Biosciences MSDS Sheet])
		+
		+	<!-- ==References==
		+	*Any papers or websites that are relevant to the use of this material
		+
		+	==Contact==
		+	*Who has experience using this material?
		+	-->
		+
		+	[[Category:Material]]

Benjamin E. Epstein at 21:19, 6 July 2008

Benjamin E. Epstein — Sun, 06 Jul 2008 21:19:08 GMT

←Older revision		Revision as of 21:19, 6 July 2008
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	* Stains cells in '''early stage of apoptosis'''		* Stains cells in '''early stage of apoptosis'''
	** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis		** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
		+	* Must be used in conjunction with vital dye (e.g. [[Propidium iodide\|PI]], [[7-AAD]]) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)

Benjamin E. Epstein: New page: * Conjugated to other fluorochromes (PE, FITC) * Stains cells in '''early stage of apoptosis''' ** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane...

Benjamin E. Epstein — Sun, 06 Jul 2008 21:16:02 GMT

New page: * Conjugated to other fluorochromes (PE, FITC) * Stains cells in '''early stage of apoptosis''' ** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane...

New page

* Conjugated to other fluorochromes (PE, FITC)
* Stains cells in '''early stage of apoptosis'''
** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis

←Older revision		Revision as of 13:29, 5 May 2009
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	-->		-->

-	[[Category:Material]]	+	[[Category:Material]] [[Category:Dye]] [[Category:Viability dye]]