Annexin-V

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Contents

Purpose

  • Stains cells in early stage of apoptosis
    • Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis

Procurement

  • Standard companies

Use

  • Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
  • Used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells from apoptotic cells
    • Annexin-V +/7-AAD +: Dead
    • Annexin-V +/7-AAD -: Apoptotic
  • BD Biosciences protocol

Safety

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