Annexin-V: Difference between revisions

Latest revision as of 06:29, 5 May 2009

Stains cells in early stage of apoptosis
- Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis

Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
Used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells from apoptotic cells
- Annexin-V +/7-AAD +: Dead
- Annexin-V +/7-AAD -: Apoptotic
BD Biosciences protocol

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-* Conjugated to other fluorochromes (PE, FITC)
+==Purpose==
 * Stains cells in '''early stage of apoptosis'''
 ** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
-* Must be used in conjunction with vital dye (e.g. [[Propidium iodide|PI]], [[7-AAD]]) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)
+==Procurement==
+* Standard companies
+==Use==
+* Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
+* Used in conjunction with vital dye (e.g. [[Propidium iodide|PI]], [[7-AAD]]) to distinguish dead cells from apoptotic cells
+** Annexin-V +/7-AAD +: Dead
+** Annexin-V +/7-AAD -: Apoptotic
+* [http://www.bdbiosciences.com/pharmingen/protocols/Annexin_V_Staining.shtml BD Biosciences protocol]
+<!-- *Include any relevant links to [[Protocols|OWW protocols]]
+*Include any relevant links to the manufacturer's protocols -->
+==Safety==
+* No significant considerations ([http://www.bdbiosciences.com/external_files/pm/doc/msds/live/web_enabled/556417-MSDS.00.pdf BD Biosciences MSDS Sheet])
+<!-- ==References==
+*Any papers or websites that are relevant to the use of this material
+==Contact==
+*Who has experience using this material?
+ -->
+[[Category:Material]] [[Category:Dye]] [[Category:Viability dye]]